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Tapso Sodium CAS:105140-25-8 Farashin Mai Masana'anta
3-[N-Tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid,Ni nesa zwitterionic buffering a cikin nau'in gishirin sodium. Ana amfani da shi sosai a cikin gwaje-gwajen biochemical da molecular sciences don kiyaye pH mai ƙarfi a cikin mafita.It yana narkewa sosai a cikin ruwa kuma yana da pKa kusa da pH na jiki, wanda hakan ya sa ya dace da aikace-aikace iri-iri kamar tsarkake furotin, gwajin enzyme, al'adar ƙwayoyin halitta, da electrophoresis. Tsarin sinadarai na musamman da kuma dacewa da tsarin halittu ya sa ya zama muhimmin sashi a cikin ka'idojin dakin gwaje-gwaje daban-daban.
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4-Nitrophenyl phosphate di(tris) gishiri CAS:68189-42-4
Gishirin Nitrophenyl phosphate di(tris) wani sinadari ne da ake amfani da shi wajen bincike da kuma gano cututtuka. Ana amfani da shi a matsayin wani abu da ake amfani da shi wajen gwajin sinadarin alkaline phosphatase. Wannan gishirin, idan aka yi amfani da shi wajen hydrolyze ta hanyar alkaline phosphatase, yana samar da samfurin launin rawaya wanda za a iya auna shi cikin sauƙi ta hanyar spectrophotometric. Ana amfani da wannan amsawar sosai a aikace-aikace daban-daban, ciki har da gwajin immunosorbent na enzyme-linked (ELISAs) da kuma gano ayyukan phosphatase. Ƙarfin launin rawaya yana daidai da aikin enzyme, wanda ke ba da damar auna matakan alkaline phosphatase daidai a cikin samfuran halittu. Gishirin Nitrophenyl phosphate di(tris) yana da karko, yana narkewa cikin ruwa, kuma yana da matuƙar tasiri, wanda hakan ya sa ya zama zaɓi mai shahara don gwaje-gwajen enzyme a cikin binciken halittu da na likitanci.
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Nitrotetrazolium blue chloride CAS:298-83-9
Nitrotetrazolium blue chloride, wanda kuma aka sani da NBT ko NBT-Cl, wani sinadari ne da ake amfani da shi a gwaje-gwajen halittu da na biochemical. Foda ce mai launin rawaya mai haske wadda take narkewa a cikin ruwa.
Ana amfani da NBT akai-akai a matsayin alamar gano kasancewar radicals na superoxide ko enzymes da ke samar da su. Yana fuskantar canjin launi idan aka rage shi ta hanyar radicals na superoxide, yana canzawa daga rawaya zuwa zurfin formazan mai launin shuɗi. Sau da yawa ana amfani da wannan amsawar a gwaje-gwaje don nazarin damuwa ta oxidative, numfashin ƙwayoyin halitta, da aikin enzyme.
Ana amfani da NBT a hanyoyi daban-daban, kamar rage yawan sinadarin nitroblue tetrazolium, don ganin yadda ake samar da superoxide a cikin ƙwayoyin halitta da kyallen takarda. Haka kuma an yi amfani da shi a cikin immunohistochemistry da histochemistry don gano da kuma gano wasu enzymes da sassan ƙwayoyin halitta.
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NSP-AS CAS:211106-69-3 Farashin Mai Masana'anta
Gishirin ciki na 3-[9-(((3-(carboxypropyl)[4-Methxylphenyl]sulfonyl)aMine)carboxyl]-10-acridiniuMyl)-1-propanesulfonate wani hadadden kwayar halitta ce da ta kunshi wani abu mai suna acridinium da aka makala a cikin gishirin ciki na propanesulfonate. Ya kunshi kungiyar carboxypropyl, kungiyar 4-Methxylphenylsulfonyl, da kuma kungiyar amine. Wannan sinadarin yana yiwuwa an hada shi ne don takamaiman amfani a fannoni kamar su sinadarai, magunguna, ko bincike.
Bugu da ƙari, NAC ta nuna alƙawarin tallafawa lafiyar hanta ta hanyar taimakawa wajen cire abubuwa masu guba, kamar acetaminophen, maganin rage radadi na yau da kullun. Hakanan yana iya samun tasirin kariya daga lalacewar hanta da shan barasa ke haifarwa.
Baya ga kaddarorin antioxidant da na taimakawa numfashi, an binciki NAC don fa'idodin da zai iya bayarwa ga lafiyar kwakwalwa. Wasu bincike sun nuna cewa yana iya yin tasiri mai kyau ga matsalolin yanayi, kamar baƙin ciki da rashin lafiyar da ke tattare da damuwa (OCD).
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L-Leucine p-nitroanilide hydrochloride CAS: 16010-98-3
L-Leucine p-nitroanilide hydrochloride wani sinadari ne da ke cikin rukunin abubuwan da aka samo daga p-nitroanilide. Foda ce ta kristal mai launin fari zuwa fari wadda ake amfani da ita a binciken sinadarai da gwaje-gwajen enzyme.
Sau da yawa ana amfani da L-Leucine p-nitroanilide hydrochloride a matsayin wani abu da ake amfani da shi wajen auna ayyukan enzymes kamar proteases da peptidases. Lokacin da waɗannan enzymes suka raba L-Leucine p-nitroanilide hydrochloride, yana fitar da p-nitroaniline, wanda za a iya gano shi ta hanyar spectrophotometric. Yawan canjin launi yana daidai gwargwado kai tsaye da aikin enzymatic, wanda ke ba da damar auna ayyukan enzyme a cikin samfuran halittu.
Wannan sinadarin yana narkewa cikin ruwa kuma ana iya narkewa cikin sauƙi a cikin ruwan da ya dace don amfani a gwaje-gwajen enzymatic. Yana da mahimmanci a adana L-Leucine p-nitroanilide hydrochloride a wuri mai sanyi da bushewa don kiyaye daidaitonsa.
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Guanidine Thiocyanate Sulfate CAS:593-84-0
Guanidine thiocyanate sulfate wani sinadari ne da aka samo daga ions na guanidine, thiocyanate, da sulfate. Ana amfani da shi sosai a fannin ilmin kwayoyin halitta da binciken sinadarai a matsayin wani abu mai sarkakiya.
Ana amfani da Guanidine thiocyanate sulfate sau da yawa a cikin ka'idojin keɓewa na DNA da RNA don wargaza membranes na ƙwayoyin halitta da sunadaran denature, wanda ke ba da damar fitar da ƙwayoyin nucleic acid cikin inganci. Yana taimakawa wajen narkewar DNA da RNA, yana ba da damar ware su da tsarkake su daga samfuran halittu daban-daban.
Saboda halayensa na chaotropic, guanidine thiocyanate sulfate yana taimakawa wajen karya haɗin hydrogen da sauran hulɗar da ba ta da covalent, yana sauƙaƙa rabuwar nucleic acid daga sunadarai, lipids, da sauran sassan tantanin halitta. Hakanan yana taimakawa wajen hana ayyukan RNase, yana tabbatar da sahihanci da tsarkin nucleic acid da aka cire..
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BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine gishiri CAS:6578-06-9
BCIP-Toluidine, wanda aka fi sani da gishirin 5-Bromo-4-chloro-3-indolylphosphate-p-toluidine, wani sinadari ne da ake amfani da shi a fannin ilmin kwayoyin halitta da kuma binciken sinadarai. Ana amfani da shi ne a matsayin wani abu da ake amfani da shi a cikin gwaje-gwajen immunosorbent na enzyme-linked (ELISAs) ko dabarun tantancewa na immunohistochemical don gano aikin alkaline phosphatase.
Idan aka raba BCIP-Toluidine da alkaline phosphatase, yana fuskantar canjin launi, yana samar da wani abu mai launin shuɗi-shuɗi. Wannan amsawar tana ba da damar gani da kuma auna ayyukan alkaline phosphatase a cikin samfura. Ana iya auna ƙarfin launin ta hanyar spectrophotometric kuma yana daidai da aikin enzyme kai tsaye.
Amfani da BCIP-Toluidine a matsayin wani abu mai kama da juna ya zama ruwan dare musamman a aikace-aikacen da suka shafi labeling na DNA ko RNA, kamar in situ hybridization (ISH) ko Southern da Northern blotting. Yana samar da wata hanya mai inganci da kuma kulawa don gano kasancewar nucleic acid da aka yi niyya a cikin samfurori.
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3-Aminophthalhydrazide CAS:521-31-3
3-Aminophthalhydrazide wani sinadari ne mai dauke da sinadarin C8H8N2O. Ana kiransa da sinadarin aminohydrazide wanda aka samo daga sinadarin phthalic acid. Wannan sinadari ya kunshi wani sinadari mai suna phthalic acid wanda aka maye gurbinsa da sinadarin hydrogen guda biyu da kuma sinadarin amino da kuma sinadarin hydrazine.
3-Aminophthalhydrazide yana da amfani daban-daban a fannin ilmin sinadarai da magunguna na halitta. Ana iya amfani da shi azaman kayan farawa wajen haɗa nau'ikan sinadarai da rini daban-daban. Bugu da ƙari, ana amfani da shi sau da yawa wajen shirya tushen Schiff, waɗanda suke da mahimmanci a cikin haɗakar kwayoyin halitta.
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4-Aminoantipyrine CAS:83-07-8
4-Aminoantipyrine, wanda aka fi sani da aminoantipyrine ko aminopyrine, wani sinadari ne na halitta da aka yi da roba. Ana amfani da shi a matsayin sinadari a fannin nazarin sinadarai da kuma masana'antar harhada magunguna. Tsarin sinadaransa ya kunshi zoben aromatic tare da rukunin amino (NH2) da kuma rukunin pyrazolone, wanda hakan ya sa ya zama sinadari mai amfani da yawa don amfani daban-daban.
A fannin nazarin sinadarai, ana amfani da 4-Aminoantipyrine sosai a matsayin sinadarin chromogenic reagent a gwaje-gwajen launi. Yana yin aiki da wasu abubuwa, kamar phenols da peroxides, don samar da hadaddun launuka waɗanda za a iya auna su ta hanyar spectrophotometric. Wannan siffa ta sa ya zama da amfani a gwaje-gwaje daban-daban, gami da tantance enzymes kamar peroxidase, glucose, da amylase.
A masana'antar harhada magunguna, an yi amfani da 4-Aminoantipyrine a tarihi a matsayin maganin rage zafi da rage kumburi. .
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TAPS-NA CAS:91000-53-2 Farashin Mai Masana'anta
Gishirin sodium na N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid, wanda aka fi sani da TAPS-Na, wani sinadari ne da ake amfani da shi a matsayin mai hana ruwa shiga a aikace-aikacen halittu da na sinadarai daban-daban. Sinadari ne na zwitterionic, ma'ana yana da caji mai kyau da mara kyau, wanda ke taimakawa wajen kiyaye pH mai karko a cikin mafita.
TAPS-Na yana da kewayon pH na kimanin 7.7 zuwa 9.1, wanda hakan ya sa ya dace da aikace-aikacen da ke buƙatar yanayin pH mai ɗan alkaline. Sau da yawa ana amfani da shi a binciken furotin da enzyme, da kuma a cikin dabarun ilimin halittar kwayoyin halitta kamar gel electrophoresis da DNA sequencing.
A matsayin gishirin sodium, TAPS-Na yana narkewa sosai a cikin ruwa, wanda ke ba da damar shirya mafita masu narkewa cikin sauƙi. An san shi da ƙarancin guba da ƙarancin tsangwama ga ƙwayoyin halitta, wanda hakan ya sa ya zama zaɓi mafi soyuwa ga gwaje-gwaje da yawa.
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5,5-Dithiobis(2-nitrobenzoic acid) CAS:69-78-3
5,5-Dithiobis (2-nitrobenzoic acid), wanda kuma aka sani da DTNB ko kuma Ellman's reagent, wani sinadari ne da ake amfani da shi a binciken sinadarai da magunguna. Foda ce mai launin rawaya, mai lu'ulu'u wadda take narkewa sosai a cikin ruwa da kuma sinadarai masu narkewa a cikin halitta.
Ana amfani da DTNB musamman don auna yawan ƙungiyoyin sulfhydryl (-SH) a cikin furotin da peptides. Wannan mahaɗin yana amsawa da thiols, wanda ke haifar da samuwar gaurayen disulfide da kuma sakin 2-nitro-5-thiobenzoate, wanda ke da launin rawaya wanda za'a iya aunawa ta hanyar spectrophotometric. Ta hanyar auna ƙarfin launin rawaya, yana yiwuwa a kimanta yawan thiols a cikin samfurin. Sau da yawa ana amfani da wannan dabarar don nazarin hulɗar furotin-protein, naɗe furotin, da halayen enzymatic waɗanda suka shafi ƙungiyoyin thiol.
Baya ga ƙididdige thiol, an yi amfani da DTNB a wasu aikace-aikace, kamar kimanta yanayin redox na ƙwayoyin halitta, nazarin daidaiton mahaɗan ƙarfe-sulfur, da kuma auna ayyukan enzymes daban-daban.
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Phenolphthalein monophosphate BIS(CYCLOHEXYLAMMONIUM) GISHIRIN CAS:14815-59-9
Gishirin Phenolphthalein monophosphate dicyclohexylammonium wani sinadari ne da aka samo daga phenolphthalein, wani ma'aunin pH da aka saba amfani da shi a dakin gwaje-gwaje. Ana amfani da nau'in gishirin dicyclohexylammonium na phenolphthalein monophosphate a matsayin wani abu mai nazarin halittu a cikin binciken halittu na biochemical da kwayoyin halitta. Ana amfani da shi galibi a cikin gwaje-gwaje ko gwaje-gwaje don gano da auna kasancewar wasu enzymes, gami da waɗanda ke da hannu a cikin ayyukan ATP (adenosine triphosphate) hydrolysis ko phosphatase. Abubuwan da ke tattare da shi sun sa ya zama kayan aiki mai amfani a cikin binciken kimiyya da suka shafi kinetics na enzyme da hanyoyin tantanin halitta..
