L-Leucine p-nitroanilide hydrochloride CAS: 16010-98-3

Ana amfani da L-Leucine p-nitroanilide hydrochloride a matsayin wani abu mai kama da sinadarai a binciken sinadarai da gwaje-gwajen enzyme don auna ayyukan enzymes daban-daban, musamman proteases da peptidases. Lokacin da waɗannan enzymes suka yanke substrate, yana fitar da p-nitroaniline, wanda za'a iya gano shi ta hanyar spectrophotometric.

Yawan canjin launi yana daidai gwargwado kai tsaye da aikin enzymatic, wanda ke ba da damar ƙididdige ayyukan enzyme a cikin samfuran halittu. Wannan ya sa L-Leucine p-nitroanilide hydrochloride kayan aiki mai amfani don nazarin motsin enzyme, tantance masu hana enzyme ko masu kunna shi, da kuma tantance tasirin yanayin gwaji ko tsoma baki akan aikin enzyme.

Jin daɗin L-Leucine p-nitroanilide hydrochloride a cikin gwaje-gwajen enzymatic daban-daban na iya bambanta, wanda ke buƙatar ingantawa ga takamaiman tsarin. Yawanci ana amfani da shi a yawan da ya kama daga 0.5 mM zuwa 5 mM, ya danganta da takamaiman enzyme da yanayin gwaji.