5,5-Dithiobis(2-nitrobenzoic acid) CAS:69-78-3

5,5-Dithiobis (2-nitrobenzoic acid), wanda kuma aka sani da DTNB ko kuma Ellman's reagent, wani sinadari ne da ake amfani da shi a binciken sinadarai da magunguna. Foda ce mai launin rawaya, mai lu'ulu'u wadda take narkewa sosai a cikin ruwa da kuma sinadarai masu narkewa a cikin halitta.

Ana amfani da DTNB musamman don auna yawan ƙungiyoyin sulfhydryl (-SH) a cikin furotin da peptides. Wannan mahaɗin yana amsawa da thiols, wanda ke haifar da samuwar gaurayen disulfide da kuma sakin 2-nitro-5-thiobenzoate, wanda ke da launin rawaya wanda za'a iya aunawa ta hanyar spectrophotometric. Ta hanyar auna ƙarfin launin rawaya, yana yiwuwa a kimanta yawan thiols a cikin samfurin. Sau da yawa ana amfani da wannan dabarar don nazarin hulɗar furotin-protein, naɗe furotin, da halayen enzymatic waɗanda suka shafi ƙungiyoyin thiol.

Baya ga ƙididdige thiol, an yi amfani da DTNB a wasu aikace-aikace, kamar kimanta yanayin redox na ƙwayoyin halitta, nazarin daidaiton mahaɗan ƙarfe-sulfur, da kuma auna ayyukan enzymes daban-daban.

Yi oda ko Nemi Farashi

Cikakken Bayani game da Samfurin

Alamun Samfura

Aikace-aikace da Tasiri

Thiol Quantification: Ana amfani da DTNB akai-akai don tantance yawan thiols a cikin samfuran furotin. Wannan bayanin yana da mahimmanci don nazarin hulɗar furotin-protein, naɗe furotin, da halayen enzymatic da suka shafi ƙungiyoyin thiol. Amsar launi na DTNB yana ba da damar auna thiols ta hanyar ma'aunin spectrophotometric.

Kimanta Yanayin Redox: An yi amfani da DTNB don tantance yanayin redox a cikin ƙwayoyin halitta, kyallen takarda, ko samfuran halittu. Ta hanyar auna canje-canje a cikin yawan thiol, masu bincike za su iya samun fahimta game da damuwa ta oxidative, aikin antioxidant, da hanyoyin siginar redox.

Gwaje-gwajen Enzyme: Yanayin amsawar DTNB na musamman tare da thiols ya sa ya zama kayan aiki mai mahimmanci don gwajin enzyme. Yawancin enzymes, kamar glutathione-S-transferases, cystathionine-γ-lyase, da thioredoxin reductase, suna dogara ne akan thiol kuma ana iya ƙididdige su ko gwada su ta amfani da DTNB.

Binciken Kwanciyar Hankali na Haɗaɗɗun Karfe da Sulfur: Ana amfani da DTNB don tantance kwanciyar hankali da amsawar mahaɗan ƙarfe da sulfur da metalloproteins. Mahaɗin zai iya gano da kuma ƙididdige kasancewar thiols da ke cikin kelancin ions na ƙarfe, yana ba da mahimman bayanai game da hulɗar ƙarfe da sulfur da kuma ilimin sunadarai na daidaitawa.

Shirya Samfurin:

Ƙarin Bayani:

Tsarin aiki	C14H8N2O8S2
Gwaji	99%
Bayyanar	Rawaya mai ƙarfi
Lambar CAS	69-78-3
shiryawa	Ƙarami da girma
Rayuwar shiryayye	Shekaru 2
Ajiya	A adana a wuri mai sanyi da bushewa
Takardar shaida	ISO.