BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine gishiri CAS:6578-06-9

BCIP-Toluidine, wanda aka fi sani da gishirin 5-Bromo-4-chloro-3-indolylphosphate-p-toluidine, wani sinadari ne da ake amfani da shi a fannin ilmin kwayoyin halitta da kuma binciken sinadarai. Ana amfani da shi ne a matsayin wani abu da ake amfani da shi a cikin gwaje-gwajen immunosorbent na enzyme-linked (ELISAs) ko dabarun tantancewa na immunohistochemical don gano aikin alkaline phosphatase.

Idan aka raba BCIP-Toluidine da alkaline phosphatase, yana fuskantar canjin launi, yana samar da wani abu mai launin shuɗi-shuɗi. Wannan amsawar tana ba da damar gani da kuma auna ayyukan alkaline phosphatase a cikin samfura. Ana iya auna ƙarfin launin ta hanyar spectrophotometric kuma yana daidai da aikin enzyme kai tsaye.

Amfani da BCIP-Toluidine a matsayin wani abu mai kama da juna ya zama ruwan dare musamman a aikace-aikacen da suka shafi labeling na DNA ko RNA, kamar in situ hybridization (ISH) ko Southern da Northern blotting. Yana samar da wata hanya mai inganci da kuma kulawa don gano kasancewar nucleic acid da aka yi niyya a cikin samfurori.