Nitrotetrazolium blue chloride CAS:298-83-9

Nitrotetrazolium blue chloride (NBT) rini ne da aka saba amfani da shi a gwaje-gwajen halittu da na biochemical. Ana amfani da shi galibi azaman alamar redox don gano da kuma hango kasancewar enzymes na dehydrogenase a cikin samfuran halittu.

Idan aka rage NBT ta hanyar enzymes na dehydrogenase, yana fuskantar canjin launi daga launin rawaya mai haske zuwa launin shuɗi mai duhu. Wannan canjin yana bawa masu bincike damar gano ayyukan da kuma inda enzymes na dehydrogenase suke a cikin kyallen takarda da ƙwayoyin halitta daban-daban. Saboda haka, ana amfani da NBT akai-akai a cikin gwaje-gwajen ganewar asali da nazarin immunohistochemical don tantance ayyukan metabolism na ƙwayoyin halitta da aikin enzymes.

Ta hanyar auna ƙarfin launin shuɗin da aka samar, masu bincike za su iya yin nazari kan aikin enzyme na dehydrogenase a adadi mai yawa, wanda yake da mahimmanci wajen fahimtar hanyoyin ƙwayoyin halitta kamar damuwa ta oxidative, numfashin ƙwayoyin halitta, da kuma metabolism na carbohydrates da lipids. Wannan bayanin yana da mahimmanci a fannoni daban-daban, gami da biochemistry, ilimin halittar ƙwayoyin halitta, da kuma binciken asibiti.