Nitrotetrazolium Blue Chloride CAS: 298-83-9

Nitrotetrazolium Blue Chloride (NBT) alama ce ta redox da aka saba amfani da ita a cikin kididdigar nazarin halittu da kwayoyin halitta.Yana da kodadde rawaya foda wanda ya juya blue lokacin da aka rage shi, yana sa ya zama mai amfani don gano gaban wasu enzymes da aikin rayuwa.

Babban sakamako na NBT shine samuwar blue formazan hazo lokacin da aka rage shi ta wasu enzymes.Wannan canjin launi yana ba da damar gano gani ko spectrophotometric na ayyukan enzyme.

NBT yana da aikace-aikace iri-iri a cikin bincike da bincike.Ga wasu daga cikin muhimman abubuwan amfaninsa:

Binciken Ayyukan Enzyme: Ana iya amfani da NBT don auna ayyukan dehydrogenases, waɗanda ke da hannu a cikin matakai kamar numfashi na salula da metabolism.Ta hanyar saka idanu akan rage NBT zuwa formazan, masu bincike zasu iya tantance ayyukan waɗannan enzymes.

Ƙimar aikin ƙwayoyin rigakafi: Ana amfani da NBT a cikin gwajin rage NBT don kimanta ayyukan fashewar numfashi na ƙwayoyin rigakafi, musamman phagocytes.Gwajin yana auna ikon waɗannan ƙwayoyin don samar da nau'in oxygen mai amsawa, wanda zai iya rage NBT kuma ya samar da hazo mai shuɗi.

Binciken Microbiology: Ana amfani da NBT a cikin ƙwayoyin cuta don nazarin ƙwayoyin cuta na ƙwayoyin cuta da kimanta ayyukan takamaiman enzymes.Alal misali, an yi amfani da shi don gano ƙwayoyin nitrate reductases ko kwayoyin formazan-forming.

Nazarin haɓakar ƙwayoyin cuta: Ragewar NBT yana ba masu bincike damar tantance ayyukan rayuwa da yuwuwar sel.Ta hanyar ƙididdige ƙarfin samfurin formazan blue, yana yiwuwa a ƙayyade adadin ƙwayoyin da za a iya amfani da su a cikin samfurin da aka ba.