ONPG CAS:369-07-3 Farashin Mai Masana'anta

Za a raba tasirin ONPG a matsayin substrate ta hanyar enzyme β-galactosidase, wanda ke haifar da sakin samfurin rawaya, o-nitrophenol. Ana iya auna wannan canjin launi ta hanyar spectrophotometric, wanda ke ba da damar ƙididdige ayyukan β-galactosidase. Amfani da ONPG galibi yana cikin kimanta bayyanar kwayoyin halitta a cikin binciken ilmin kwayoyin halitta da ƙwayoyin cuta. Ana amfani da shi galibi don auna ayyukan β-galactosidase a matsayin mai ba da rahoto don nazarin bayyanar kwayoyin halitta, musamman a cikin ƙwayoyin cuta kamar E. coli. Kwayoyin lacZ, waɗanda ke ɓoye β-galactosidase, galibi ana amfani da su azaman alamar nazarin bayyanar kwayoyin halitta, saboda ana iya haifar da bayyanar ta ta hanyar takamaiman yanayi ko kuma masu haɓaka takamaiman sunadaran suna sarrafawa. Gwajin ONPG yana ba da hanya mai sauƙi da aminci don tantance matakin bayyanar kwayoyin halitta ta hanyar auna ayyukan β-galactosidase. Ana amfani da wannan gwajin sosai a aikace-aikace daban-daban, kamar nazarin ayyukan mai haɓaka kwayoyin halitta, daidaita kwayoyin halitta, da hulɗar furotin da furotin. Bugu da ƙari, ana iya amfani da shi don tantance motsin enzyme da kuma kimanta tasirin maye gurbi ko jiyya akan aikin enzyme.