4-Nitrophenyl beta-D-galactopyranoside CAS:200422-18-0

Tasiri: ONPG wani abu ne da ake amfani da shi musamman don gano kasancewar da kuma aikin enzyme β-galactosidase. Lokacin da enzyme β-galactosidase yake akwai kuma yana aiki, yana raba ONPG zuwa samfura biyu: o-nitrophenol da kuma galactose derivative. 'Yantar da o-nitrophenol yana haifar da canjin launin rawaya, wanda za'a iya aunawa ta amfani da na'urar auna hoto.

Aikace-aikace: ONPG yana da aikace-aikace da yawa a cikin ilimin halittar kwayoyin halitta da binciken sinadarai na biochemistry:

Tabbatar da aikin β-galactosidase: Ana amfani da ONPG akai-akai don aunawa da kuma auna ayyukan enzyme na β-galactosidase. Ana iya auna yawan samuwar o-nitrophenol, wanda yake daidai da aikin enzyme kai tsaye, ta hanyar spectrophotometric.

Bayyanar Halitta da Ka'idojin Halitta: Sau da yawa ana amfani da ONPG a gwaje-gwajen da suka shafi bayyanar kwayar halitta da nazarin ka'idoji. Ana amfani da shi akai-akai a cikin gwaje-gwajen furotin na haɗuwa, kamar tsarin haɗakar lacZ da aka saba amfani da shi, don nazarin bayyanar kwayoyin halitta a ƙarƙashin ikon takamaiman masu haɓaka. Ayyukan beta-galactosidase da aka auna ta amfani da ONPG yana ba da haske game da matakin bayyanar kwayar halitta.

Duba ayyukan β-galactosidase: Ana iya amfani da ONPG a matsayin hanyar tantance launi a cikin fasahar DNA mai haɗawa don gano kasancewar da aikin kwayar halittar LacZ, wacce ke ƙunshe da β-galactosidase. Wannan hanyar tantancewa tana taimakawa wajen gano ƙwayoyin cuta waɗanda ke ɗauke da kwayar halittar da ake sha'awa.

Nazarin kinetics na enzyme: ONPG kuma yana da amfani wajen nazarin kinetics na enzyme β-galactosidase. Ta hanyar auna saurin amsawar enzyme-substrate a yawan substrate daban-daban, yana yiwuwa a tantance sigogin kinetic kamar su Michaelis-Menten constants (Km) da matsakaicin yawan amsawa (Vmax).