Nitrotetrazolium Blue Chloride CAS:298-83-9

Nitrotetrazolium Blue Chloride (NBT) wata alama ce ta redox da aka saba amfani da ita a gwaje-gwajen halittu da na sinadarai. Foda ce mai launin rawaya mai haske wadda ke canzawa zuwa shuɗi idan aka rage ta, wanda hakan ke sa ta zama da amfani wajen gano kasancewar wasu enzymes da ayyukan metabolism.

NBT yana yin aiki tare da masu ɗaukar electrons da enzymes kamar dehydrogenases, waɗanda ke da hannu a cikin ayyukan ƙwayoyin halitta daban-daban. Lokacin da aka rage NBT ta hanyar waɗannan enzymes, yana samar da wani abu mai launin shuɗi mai kama da formazan, wanda ke ba da damar gano gani ko spectrophotometric.

Ana amfani da wannan sinadarin a gwaje-gwaje kamar gwajin rage NBT, inda ake amfani da shi don tantance aikin fashewar numfashi na ƙwayoyin garkuwar jiki. Haka kuma ana iya amfani da shi don nazarin ayyukan enzymes da hanyoyin rayuwa a cikin bincike da suka shafi damuwa ta oxidative, yuwuwar ƙwayoyin halitta, da bambancewar ƙwayoyin halitta.

NBT ta sami aikace-aikace a fannoni daban-daban, ciki har da ilimin ƙwayoyin cuta, ilimin rigakafi, da kuma ilimin halittar ƙwayoyin halitta. Yana da amfani mai yawa, yana da kwanciyar hankali, kuma yana da sauƙin amfani, wanda hakan ya sa ya zama zaɓi mai shahara ga yawancin ka'idojin gwaji.

Yi oda ko Nemi Farashi

Cikakken Bayani game da Samfurin

Alamun Samfura

Aikace-aikace da Tasiri

Babban tasirin NBT shine samuwar wani abu mai launin shuɗi mai kama da formazan lokacin da wasu enzymes suka rage shi. Wannan canjin launi yana ba da damar gano ayyukan enzyme na gani ko na spectrophotometric.

NBT tana da amfani iri-iri a fannin bincike da bincike. Ga wasu daga cikin manyan amfaninta:

Gwaje-gwajen ayyukan enzyme: Ana iya amfani da NBT don auna ayyukan dehydrogenases, waɗanda ke da hannu a cikin ayyuka kamar numfashin ƙwayoyin halitta da metabolism. Ta hanyar sa ido kan rage NBT zuwa formazan, masu bincike za su iya tantance ayyukan waɗannan enzymes.

Kimanta aikin ƙwayoyin rigakafi: Ana amfani da NBT akai-akai a gwajin rage NBT don tantance aikin fashewar numfashi na ƙwayoyin rigakafi, musamman phagocytes. Gwajin yana auna ikon waɗannan ƙwayoyin halitta na samar da nau'in iskar oxygen mai amsawa, wanda zai iya rage NBT da kuma samar da wani abu mai launin shuɗi.

Binciken Kwayoyin Halitta: Ana amfani da NBT a fannin ƙwayoyin halitta don nazarin tsarin metabolism na ƙwayoyin cuta da kuma tantance ayyukan takamaiman enzymes. Misali, an yi amfani da shi don gano ƙwayoyin cuta na nitrate reductases ko ƙwayoyin cuta masu samar da formazan.

Nazarin wanzuwar ƙwayoyin halitta: Ragewar NBT yana ba masu bincike damar tantance ayyukan metabolism da kuma wanzuwar ƙwayoyin halitta. Ta hanyar ƙididdige ƙarfin samfurin formazan mai launin shuɗi, yana yiwuwa a tantance adadin ƙwayoyin halitta masu wanzuwa a cikin samfurin da aka bayar.

Samfurin Samfuri

Shirya Samfurin:

Ƙarin Bayani:

Tsarin aiki	C40H30ClN10O6+
Gwaji	99%
Bayyanar	Foda mai launin rawaya
Lambar CAS	298-83-9
shiryawa	Ƙarami da girma
Rayuwar shiryayye	Shekaru 2
Ajiya	A adana a wuri mai sanyi da bushewa
Takardar shaida	ISO.