5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide wani sinadari ne da ake amfani da shi a cikin nazarin sinadarai daban-daban, musamman don gano da kuma ganin ayyukan enzymes. Wani sinadari ne da za a iya samar da hydrolyzed ta hanyar takamaiman enzymes, wanda ke haifar da fitowar samfurin mai launi ko fluorescent.

Ana amfani da wannan sinadari a gwaje-gwaje don gano kasancewar da kuma ayyukan enzymes kamar beta-galactosidase da beta-glucuronidase. Waɗannan enzymes suna raba ƙungiyoyin acetyl da glucosaminide daga substrate, wanda ke haifar da samuwar chromophore mai shuɗi ko kore.

Tsarin musamman na 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide yana ba da damar ganowa da kuma ƙididdige ayyukan enzyme cikin sauƙi. Amfani da shi a cikin fasahohin gwaji daban-daban, gami da histochemistry, immunohistochemistry, da gwaje-gwajen da aka yi bisa ƙwayoyin halitta, ya ba da gudummawa ga fahimtar ayyukan enzyme sosai.

Yi oda ko Nemi Farashi

Cikakken Bayani game da Samfurin

Alamun Samfura

Aikace-aikace da Tasiri

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) wani sinadari ne da ake amfani da shi wajen gano ayyukan beta-glucuronidase (GUS). GUS wani sinadari ne da ake samu a cikin halittu daban-daban, ciki har da ƙwayoyin cuta, shuke-shuke, da dabbobi masu shayarwa. Ana yawan amfani da X-Gluc a cikin gwaje-gwajen masu rahoto na GUS da binciken ilmin halitta na kwayoyin halitta.

Babban amfani da X-Gluc shine a cikin dabarun tabo na histochemical, inda zai iya hango bayyanar da aikin enzyme na GUS. Wannan substrate yana iya shiga cikin tantanin halitta kuma GUS yana samar da hydrolyzed, wanda ke haifar da samuwar wani abu mai launin shuɗi ko samfurin da ba ya narkewa. Wannan tabo mai launin shuɗi yana bawa masu bincike damar gano da kuma gano ayyukan GUS a cikin ƙwayoyin halitta, kyallen takarda, da dukkan halittu.

Ana iya amfani da X-Gluc a gwaje-gwajen adadi don auna aikin enzyme na GUS. Ƙarfin launin shuɗi ko adadin samfurin da aka samar za a iya danganta shi da matakin bayyanar GUS ko aikin enzyme ɗinsa.

Bugu da ƙari, an yi amfani da X-Gluc a cikin binciken kwayoyin halittar tsirrai don nazarin bayyanar kwayoyin halitta, ayyukan haɓaka, da kuma canza tsire-tsire. An kuma yi amfani da shi a cikin tsarin ƙwayoyin cuta don yin cloning da gano furotin na haɗin GUS.

Shirya Samfurin:

Ƙarin Bayani:

Tsarin aiki	C16H18BrClN2O6
Gwaji	99%
Bayyanar	Foda fari
Lambar CAS	4264-82-8
shiryawa	Ƙarami da girma
Rayuwar shiryayye	Shekaru 2
Ajiya	A adana a wuri mai sanyi da bushewa
Takardar shaida	ISO.