3,3 ,5,5 -Tetramethylbenzidine dihydrochloride hydrate CAS:207738-08-7

3,3',5,5'-Tetramethylbenzidine dihydrochloride (TMB) wani abu ne da ake amfani da shi sosai a cikin gwaje-gwajen immunosorbent da ke da alaƙa da enzyme (ELISAs) da sauran gwaje-gwajen biochemical. Lokacin da enzyme horseradish peroxidase (HRP) ya shafa TMB a gaban hydrogen peroxide, yana samar da launin shuɗi. Ana amfani da wannan canjin launi don gano kasancewar ƙwayoyin rigakafi ko wasu sunadarai masu alama da HRP, sau da yawa yana nuna kasancewar wani takamaiman antigen a cikin samfurin. Launin shuɗin da amsawar ta haifar yawanci ana auna shi ta amfani da na'urar aunawa, wanda ke ba da damar auna ƙarfin canjin launi kuma yana ba da hanyar daidaita ƙarfin launi da adadin ƙwayoyin da aka yi niyya a cikin samfurin. TMB yana da aikace-aikace da yawa a cikin gwaje-gwajen ganewar asali, bincike, da haɓaka magunguna, musamman a cikin gano ƙwayoyin rigakafi, hormones, cytokines, da sauran ƙwayoyin halitta. Jin daɗinsa, kwanciyar hankali, da sauƙin amfani sun sa ya zama kayan aiki mai mahimmanci a cikin saitunan dakin gwaje-gwaje daban-daban don gano da ƙididdige takamaiman ƙwayoyin halitta.