SYBR GREEN I Nucleic Acid Gel Tabo CAS:163795-75-3

Gener na DNA electrophoresis: Ana ƙara tabon SYBR kore I a cikin gel ɗin DNA bayan electrophoresis, kuma idan aka fallasa shi ga hasken UV, yana ɗaurewa da madaurin DNA, yana samar da haske mai haske. Wannan hanyar fenti tana taimakawa wajen hango da kuma nazarin gutsuttsuran DNA da aka raba bisa girmansu.

PCR mai ƙididdigewa (qPCR): Ana amfani da SYBR Green I a cikin qPCR don sa ido kan haɓaka jerin DNA ko RNA a ainihin lokaci. Rini yana ɗaure da DNA mai layi biyu da aka samar yayin haɓaka PCR, kuma ana auna ƙaruwar ƙarfin haske a kowane zagayen haɓakawa, wanda ke ba da damar ƙididdige samfurin nucleic acid na farko.

Ana iya amfani da SYBR Green I don electrophoresis na gel na RNA, wanda ke ba da damar gani da kuma nazarin gutsuttsuran RNA. Yana da amfani don kimanta ingancin RNA da kuma nazarin matakan bayyanar RNA.

Lakabi da gano sinadarin Nucleic acid: Ana iya amfani da SYBR Green I don yiwa sinadarin nucleic acid alama da hasken rana. Ana iya cimma wannan ta hanyar haɗa rini yayin haɗa sinadarin nucleic acid ko ta amfani da dabarun bayan an yi masa alama. Sannan ana iya ganin sinadarin nucleic acid ɗin da aka yi wa alama da kuma gano shi ta amfani da tsarin daukar hoton fluorescent.