PMSF CAS:329-98-6 Farashin Mai Masana'anta

Tasiri:

PMSF wani maganin hana sinadarin serine protease ne wanda ke ɗaurewa zuwa wurin da sinadarin serine proteases ke aiki ba tare da wata matsala ba, ta haka ne zai toshe ayyukansu.

Yana canza ragowar serine ta hanyar haɗin covalent, wanda ke haifar da rashin aiki na enzymes.

PMSF na iya kare sunadaran daga lalacewa ta hanyar hana ayyukan protease, musamman sunadarai masu ɗauke da sinadarin serine.

Aikace-aikace:

Binciken sinadarai: Ana amfani da PMSF a lokacin tsarkake furotin da kuma keɓancewa don hana lalacewar furotin. Yana taimakawa wajen kiyaye daidaito da kwanciyar hankali na furotin da aka yi niyya.

Hana Enzyme: PMSF muhimmin sinadari ne don dakatar da aikin proteases na serine yayin gwajin enzyme ko nazarin sinadarai. Ta hanyar toshe ayyukan waɗannan proteases, masu bincike za su iya bincika rawar da serine proteases ke takawa a cikin hanyoyin halitta daban-daban.

Nazarin Proteolysis: Ana iya amfani da PMSF don sarrafa ko daidaita ayyukan protease a cikin ƙwayoyin halitta ko abubuwan da aka cire. Wannan yana da amfani don nazarin abubuwan da ke faruwa na proteolytic da ke tattare da ayyukan ƙwayoyin halitta ko lokacin da ake buƙatar daidaita proteolysis a cikin saitin gwaji.

Hana protease gaba ɗaya: Duk da cewa PMSF ta fi mayar da hankali kan proteases na serine, tana kuma iya yin tasiri mai ƙarfi ga wasu cysteine ​​proteases. Ana amfani da ita azaman maganin hana protease gaba ɗaya lokacin aiki da samfuran furotin don rage lalacewar protease.