P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

P-Nitrophenyl beta-D-lactopyranoside, wanda kuma aka sani da PNPG, wani sinadari ne da ake amfani da shi a gwaje-gwajen enzymatic don auna ayyukan beta-galactosidase, wani enzyme da ke da hannu a cikin metabolism na carbohydrate. PNPG wani sinadari ne na roba wanda beta-galactosidase zai iya raba shi, wanda ke haifar da sakin samfurin mai launin rawaya. Ana iya auna girman hydrolysis na substrate ta hanyar spectrophotometric ta hanyar auna shan samfurin a wani takamaiman tsayin tsayi. Wannan yana bawa masu bincike damar tantance aiki da motsin beta-galactosidase a cikin yanayi daban-daban, kamar nazarin aikin enzyme, tantance masu hana enzyme ko masu kunna enzyme, ko kimanta tasirin maye gurbi akan aikin enzyme.

Yi oda ko Nemi Farashi

Cikakken Bayani game da Samfurin

Alamun Samfura

Aikace-aikace da Tasiri

Gano ayyukan beta-galactosidase: Ana amfani da PNPG a gwaje-gwaje don auna aikin beta-galactosidase, wani enzyme wanda ke haɓaka hydrolysis na lactose zuwa glucose da galactose. Hydrolysis na PNPG ta hanyar beta-galactosidase yana fitar da kwayar p-nitrophenol (pNP), wanda za'a iya gano shi ta hanyar spectrophotometric saboda launin rawayarsa.

Binciken masu hana enzyme da masu kunna enzyme: Ana iya amfani da PNPG a cikin gwaje-gwaje masu yawa don gano mahaɗan da ke daidaita aikin beta-galactosidase. Ta hanyar auna saurin hydrolysis na PNPG a gaban mahaɗan gwaji daban-daban, masu bincike za su iya gano masu hana enzyme waɗanda ke rage ayyukan enzyme ko masu kunna enzyme waɗanda ke haɓaka aikin enzyme.

Nazarin motsin enzyme: Ruwan PNGG da beta-galactosidase ke fitarwa ya biyo bayan motsin Michaelis-Menten, wanda ke ba masu bincike damar tantance muhimman sigogin enzyme kamar matsakaicin saurin amsawa (Vmax) da kuma madaidaitan Michaelis (Km). Wannan bayanin yana taimakawa wajen fahimtar kusancin substrate na enzyme da ingancin catalytic.

Aikace-aikacen ilmin halittar kwayoyin halitta: Beta-galactosidase, wanda ke raba PNPG, ana amfani da shi azaman kwayar halittar mai ba da rahoto a fannin ilmin halittar kwayoyin halitta. Sau da yawa ana amfani da sinadarin PNPG don gano da kuma hango bayyanar kwayar halittar mai ba da rahoto, yana samar da hanya mai sauƙi da kulawa don tantance bayyanar kwayar halitta a cikin tsarin gwaji daban-daban.

Samfurin Samfuri

Shirya Samfurin:

Ƙarin Bayani:

Tsarin aiki	C18H25NO13
Gwaji	99%
Bayyanar	Foda fari
Lambar CAS	4419-94-7
shiryawa	Ƙarami da girma
Rayuwar shiryayye	Shekaru 2
Ajiya	A adana a wuri mai sanyi da bushewa
Takardar shaida	ISO.