P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

Gano ayyukan beta-galactosidase: PNPG yawanci ana amfani dashi a cikin gwaje-gwaje don auna ayyukan beta-galactosidase, wani enzyme wanda ke haifar da hydrolysis na lactose cikin glucose da galactose.Halin hydrolysis na PNPG ta beta-galactosidase yana fitar da kwayoyin p-nitrophenol (pNP), wanda za'a iya gano shi ta hanyar kallo saboda launin rawaya.

Nunawa ga masu hana enzyme da masu kunnawa: Ana iya amfani da PNPG a cikin babban aikin nunawa don gano mahadi waɗanda ke daidaita ayyukan beta-galactosidase.Ta hanyar auna ƙimar PNPG hydrolysis a gaban mahaɗan gwaji daban-daban, masu bincike zasu iya gano masu hanawa waɗanda ke rage ayyukan enzyme ko masu kunnawa waɗanda ke haɓaka ayyukan enzyme.

Nazarin kinetics na enzyme: Hydrolysis na PNPG ta beta-galactosidase ya biyo bayan Michaelis-Menten kinetics, kyale masu bincike su tantance mahimman sigogin enzyme kamar matsakaicin saurin amsawa (Vmax) da Mika'is akai-akai (Km).Wannan bayanin yana taimakawa wajen fahimtar ƙaƙƙarfan ƙayyadaddun ƙayyadaddun ƙwayar enzyme da ingantaccen aikin catalytic.

Aikace-aikacen ilimin halitta na ƙwayoyin cuta: Beta-galactosidase, wanda ke raba PNPG, ana amfani da shi azaman mai ba da rahoto a cikin ilimin halitta.Ana amfani da ma'aunin PNPG sau da yawa don ganowa da kuma ganin yanayin bayyanar halittar mai ba da rahoto, yana ba da hanya mai sauƙi da mahimmanci don tantance maganganun kwayoyin halitta a cikin tsarin gwaji daban-daban.