ONPG CAS: 369-07-3 Farashin Mai samarwa

Tasirin ONPG a matsayin madaidaicin shine za a raba shi ta hanyar enzyme β-galactosidase, wanda ke haifar da sakin samfurin rawaya, o-nitrophenol.Za'a iya auna wannan canjin launi na spectrophotometrically, yana ba da izinin ƙididdige ayyukan β-galactosidase. Aikace-aikacen ONPG shine da farko a cikin kima na maganganun kwayoyin halitta a cikin kwayoyin halitta da bincike na microbiology.Ana amfani da shi don auna aikin β-galactosidase a matsayin mai ba da rahoto don nazarin maganganun kwayoyin halitta, musamman a cikin kwayoyin cuta kamar E. coli.Halin lacZ, wanda ke ɓoye β-galactosidase, ana amfani dashi akai-akai azaman alama don nazarin maganganun kwayoyin halitta, kamar yadda za'a iya haifar da maganganunsa ta wasu yanayi ko sarrafawa ta wasu masu tallatawa. Ƙididdigar ONPG yana ba da hanya mai dacewa da abin dogara don tantance matakin matakin. Maganar kwayoyin halitta ta hanyar auna aikin β-galactosidase.Ana amfani da wannan ƙididdiga sosai a aikace-aikace daban-daban, kamar nazarin ayyukan haɓakawa, ƙa'idodin tsarin halitta, da hulɗar furotin-protein.Bugu da ƙari, ana iya amfani da shi don ƙayyade kinetics enzyme da kimanta tasirin maye gurbi ko jiyya akan ayyukan enzyme.