Akwai jarumai da yawa da ba a taɓa jin labarinsu ba a kusa da mu, waɗanda suka yi kama da talakawa, amma a zahiri suna ba mu gudummawa sosai a hankali. Proteinase K shine "jarumin da ba a taɓa jin labarinsa ba" a masana'antar binciken ƙwayoyin halitta, kodayake idan aka kwatanta da "babba da ƙarfi" a masana'antar, proteinase K ba shi da mahimmanci sosai har muka daɗe muna mantawa da mahimmancinsa. Tare da barkewar sabuwar annobar kambi, buƙatar proteinase K ta ƙaru, kuma wadatar da ake samu a gida da waje ta yi nisa da yawan amfani da shi, kuma kowa ya fahimci cewa proteinase K yana da matuƙar muhimmanci.
Menene amfanin Proteinase K?
Proteinase K wani sinadari ne na serine protease wanda ke da aikin enzyme na proteolytic kuma yana iya kula da aiki a wurare daban-daban (pH (4-12.5), mai riƙe da gishiri mai yawa, zafin jiki mai yawa na 70°C, da sauransu). Bugu da ƙari, aikin proteinase K ba ya hana SDS, urea, EDTA, guanidine hydrochloride, guanidine isothiocyanate, da sauransu, kuma wani adadin sabulun wanke-wanke na iya haɓaka aikin proteinase K. A cikin maganin likita (ƙwayar cuta da ƙwayoyin cuta), abinci (tausasa nama), fata (tausasa gashi), yin ruwan inabi (bayyana barasa), shirya amino acid (fuka-fukan da suka lalace), cire nucleic acid, in situ hybridization, da sauransu, proteinase K Akwai aikace-aikace. Aikace-aikacen da aka fi amfani da shi shine cire nucleic acid.
Proteinase K na iya samar da enzyme ga dukkan nau'ikan furotin a cikin samfurin, gami da waɗancan histones waɗanda ke daure da ƙwayoyin nucleic acid, ta yadda za a iya fitar da ƙwayoyin nucleic acid daga samfurin kuma a sake su cikin abin da aka cire, wanda hakan zai sauƙaƙa mataki na gaba na cirewa da tsarkakewa. A cikin gano ƙwayoyin nucleic acid na ƙwayoyin cuta, proteinase K yana ɗaya daga cikin mahimman abubuwan da ke cikin maganin samfurin ƙwayoyin cuta. Proteinase K na iya fashewa da kashe furotin na ƙwayar cuta, wanda ya fi aminci a lokacin jigilar kaya da ganowa; Bugu da ƙari, proteinase K kuma yana iya lalata RNase yana hana lalacewar RNA na ƙwayar cuta kuma yana sauƙaƙe gano ƙwayoyin nucleic acid.
Shaharar proteinase K cikin dare ɗaya
Ko a fannin binciken kimiyya ko a fannin IVD, fitar da sinadarin nucleic acid shine gwaji mafi sauƙi, don haka proteinase K koyaushe yana da matuƙar muhimmanci. Duk da haka, a baya, proteinase K ba a san shi sosai ba kamar rawar da yake takawa. Babban ɓangare na wannan shine saboda alaƙar wadata da buƙata ta proteinase K ta kasance mai ƙarfi sosai. Mutane kaɗan ne za su yi tunanin cewa samar da proteinase K zai zama matsala.
Da barkewar sabuwar annobar kambi, buƙatar gwajin nucleic acid ta ƙaru. Ya zuwa ƙarshen watan Yunin 2020, China ta kammala gwaje-gwajen sabbin kambi kusan miliyan 90, kuma wannan adadin ya fi tayar da hankali a duniya baki ɗaya. A cikin gwaje-gwajen cire ƙwayoyin nucleic acid, yawan aikin proteinase K shine kusan 50-200 μg/mL. Gabaɗaya, yana ɗaukar kimanin μg 100 na proteinase K don cire samfurin nucleic acid. A zahiri, don ƙara ingancin cire ƙwayoyin nucleic acid, sau da yawa za a yi amfani da Proteinase K a cikin ƙarin adadin. Gano ƙwayoyin nucleic acid na sabon coronavirus ya kawo yawan buƙatar proteinase K mai yawa. Daidaiton wadata da buƙata na proteinase K ya lalace da sauri, kuma proteinase K ya zama muhimmin kayan rigakafin annoba cikin dare ɗaya.
Matsalolin da ke tattare da samar da sinadarin proteinase K
Duk da cewa da ci gaban annobar, mutane sun daraja muhimmancin furotinase K, abin kunya ne cewa saboda ƙarancin sinadarin proteinase K da ya wuce kima, ƙananan kamfanoni na cikin gida ne suka shiga cikin samar da sinadarin proteinase K. Lokacin da mutane ke son kafa samar da sinadarin proteinase K A lokacin samar da shi, an gano cewa sinadarin proteinase K wani sinadari ne na musamman. Yana da matuƙar wahala a faɗaɗa ƙarfin samar da sinadarin proteinase K cikin ɗan gajeren lokaci.
Samar da sinadarin proteinase K mai yawa yana fuskantar matsaloli kamar haka
1. Ƙarancin magana
Proteinase K ba zai iya lalata yawancin furotin ba musamman kuma ya haifar da mummunan guba ga ƙwayar mai masaukin baki. Saboda haka, matakin bayyanar proteinase K gabaɗaya yana da ƙasa sosai. Binciken tsarin bayyanar da nau'ikan da ke nuna proteinase K sosai gabaɗaya yana buƙatar tsawon zagaye.
2. Ragowar launuka da kuma sinadarin nucleic acid
Babban fermentation yana haifar da adadi mai yawa na launuka da kuma ragowar nucleic acid. Yana da wuya a cire waɗannan ƙazanta ta hanyar sauƙin tsarkakewa, kuma tsarkakewa mai rikitarwa yana ƙara farashi kuma yana rage saurin murmurewa.
3. Rashin kwanciyar hankali
Proteinase K bai da ƙarfi sosai, yana iya samar da enzyme da kansa, kuma yana da wuya a adana shi a yanayin zafi na 37°C na dogon lokaci ba tare da wani abu mai kariya ba.
4. Mai sauƙin fashewa
Lokacin shirya foda da aka daskare na proteinase K, domin tabbatar da cewa sinadarin proteinase K mai ƙarfi a cikin foda da aka daskare yana da girma, ya zama dole a ƙara wani abu mai kariya da aka daskare a babban taro, amma lokacin da yawan sinadarin proteinase K ya kai 20mg/mL ko sama da haka, yana da sauƙi. Tarin yana samar da wani abu mai fashewa, wanda ke kawo manyan matsaloli ga daskare-busar da proteinase K mai yawan abu mai ƙarfi.
5. Babban jari
Proteinase K yana da ƙarfin aikin protease kuma yana iya samar da hydrolyzes ga sauran proteases a cikin dakin gwaje-gwaje. Saboda haka, proteinase K yana buƙatar fannoni na musamman na samarwa, kayan aiki, da ma'aikata don bincike da haɓakawa da samarwa.
Maganin proteinase K na XD BIOCHEM
XD BIOCHEM yana da tsarin bayyanar furotin mai girma da tsarkakewa, kuma yana da ƙwarewa mai yawa a cikin bayyanawa da tsarkake sunadaran sake haɗawa da inganta hanyoyin samarwa. Ta hanyar ƙirƙirar ƙungiyar bincike da haɓakawa cikin sauri, an shawo kan babban tsarin samar da furotinase K. Fitar da foda busasshe a kowane wata ya fi KG 30. Samfurin yana da ingantaccen aiki, babban aiki na musamman na enzyme, kuma babu ragowar cytochrome da nucleic acid mai masaukin baki. Barka da zuwa tuntuɓar XD BIOCHEM Sami fakitin gwaji (Imel:sales@xdbiochem.comLambar waya: +86 513 81163739).
Maganin fasaha na XD BIOCHEM sun haɗa da
Ta amfani da haɗin plasmid mai kwafi da yawa, ana zaɓar nau'ikan bayyanar cututtuka masu girma tare da matakin bayyanar cututtuka na 8g/L, wanda ke shawo kan matsalar ƙarancin matakin bayyanar cututtuka na proteinase K.
Ta hanyar kafa tsarin tsarkakewa mai matakai da yawa, an cire ragowar cytochrome da nucleic acid na proteinase K cikin nasara ƙasa da ƙimar da aka saba.
Ta hanyar tantancewa mai ƙarfi na tsarin kariya, an zaɓi ma'ajiyar da za ta iya adana furotin K a zafin jiki na 37°C.
Tacewar ma'ajiyar sinadarai (screening buffers) tana shawo kan matsalar cewa sinadarin proteinase K yana da sauƙin taruwa da kuma haifar da shi a cikin yawan sinadarin, kuma tana shimfida harsashin busar da sinadarin proteinase K mai ƙarfi.
Samfurin XD BIOCHEM proteinase K
Gwajin kwanciyar hankali na XD BIOCHEM proteinase K: ba za a sami wani muhimmin canji a cikin aiki ba bayan kwana 80 a zafin ɗaki
Gwajin kwanciyar hankali na XD BIOCHEM proteinase K: ba za a sami wani muhimmin canji a cikin aiki ba bayan kwana 80 a zafin ɗaki.
Kwatanta tasirin fitar da sinadarin nucleic acid na sinadarin XD BIOCHEM proteinase K da samfuran da ke fafatawa. A tsarin fitar da sinadarin nucleic acid, ana amfani da sinadarin XD BIOCHEM da sinadarin proteinase K masu fafatawa bi da bi. Ingancin fitar da sinadarin XD BIOCHEM proteinase K ya fi girma kuma ƙimar Ct na kwayar halittar da aka yi niyya ta yi ƙasa.
Lokacin Saƙo: Disamba-31-2021