Dithiothreitol (DTT), CAS: 3483-12-3 wani sabon nau'in ƙari mai kore

Dithiothreitol (DTT), CAS: 3483-12-3, a matsayin wani sinadari na binciken kimiyya da ake amfani da shi sosai, galibi ana amfani da shi azaman sinadari mai rage sinadarin sulfhydryl DNA, sinadari mai hana karewa, da kuma rage haɗin disulfide a cikin sunadaran. Sabon nau'in sinadari mai kore yana taka muhimmiyar rawa wajen inganta aikin batir.

Dithiothreitol (DTT) wani sinadari ne mai ƙarfi na rage yawan sinadarin, kuma raguwarsa ta fi faruwa ne saboda daidaiton yanayin zoben mai membobi shida (wanda ke ɗauke da haɗin disulfide) a yanayin iskar oxygen. Rage haɗin disulfide na yau da kullun ta hanyar dithiothreitol ya ƙunshi halayen musayar haɗin sulfhydryl-disulfide guda biyu a jere. Ƙarfin ragewar dithiothreitol (DTT) yana shafar ƙimar pH, kuma yana iya taka rawa ne kawai lokacin da ƙimar pH ta fi 7. Wannan saboda anions na thiolate da aka cire kawai suna amsawa, yayin da mercaptans ba sa amsawa, kuma pKa na ƙungiyoyin mercapto gabaɗaya shine 8.3.

Ana amfani da Dithiothreitol (DTT) akai-akai don rage haɗin disulfide na ƙwayoyin furotin da polypeptides. Yawanci ana amfani da shi azaman wakili mai kariya daga furotin sulfhydryl kuma ana amfani da shi a shirye-shiryen rigakafi don hana ragowar cysteine ​​​​protein cysteine ​​​​kamuwa da disulfides na intramolecular. key. A cikin tsarin gano nucleic acid, dithiothreitol (DTT) na iya lalata haɗin disulfide a cikin furotin RNase, cire RNase, da sauƙaƙe gudanar da gwaje-gwaje kamar gina ɗakin karatu na RNA da haɓaka RNA. Ana kuma amfani da Dithiothreitol (DTT) azaman maganin rigakafi don kare ƙwayoyin halitta da kyallen takarda, azaman mai kare rediyo, da sauransu.

Duk da haka, dithiothreitol (DTT) sau da yawa ba ya iya rage haɗin disulfide da ke cikin tsarin furotin (wanda ba za a iya samunsa ba). Rage irin waɗannan haɗin disulfide sau da yawa yana buƙatar cire furotin daga jiki da farko.

Domin hana tasirin batirin lithium-sulfur da kuma inganta aikin lantarki na batirin lithium-sulfur, yi ƙoƙarin amfani da dithiothreitol (DTT) a matsayin mai yankewa don yanke polysulfides masu girma don hana su narkewa. Threitol (DTT) an haɗa shi cikin takarda mai bango da yawa na carbon nanotubes (MWCNTs) don shirya layin DTT. Ana sanya layin DTT tsakanin takardar lantarki mai kyau da mai raba rabin sel na maɓallin lithium-sulfur, da kuma yawan saman takardar lantarki mai kyau mai ɗauke da sulfur. Kimanin 2mg/cm2. Sakamakon lura da SEM ya tabbatar da cewa DTT ya bazu a saman da kuma ɓoye na takardar MWCNTs. Sakamakon gwajin electrochemical ya nuna cewa batirin lithium-sulfur tare da tsarin sandwich na DTT yana da ƙarfin fitarwa na farko na 1288 mAh/g a ƙimar 0.05C. A karon farko, ingancin coulombic ya kusa kusan kashi 100%, kuma takamaiman ƙarfin aiki yayin caji da fitarwa a 0.5C, 2C, da 4C ya kai 650mAh/g, 600mAh/g, da 410mAh/g, bi da bi. Gabatar da tsarin sandwich na DTT zai iya rage yawan polysulfides masu inganci. Yana hana shi ƙaura zuwa ga electrode na lithium, ta haka yana hana tasirin shuttle da inganta daidaiton zagayowar da ingancin coulomb na batirin lithium-sulfur.

Ya kamata a lura cewa dithiothreitol (DTT) abu ne mai guba. Misali, a gaban karafa masu canzawa, dithiothreitol (DTT) na iya haifar da lalacewar oxidative ga kwayoyin halittu. A lokaci guda, dithiothreitol (DTT) ) Hakanan yana iya haɓaka gubar wasu mahadi da ke ɗauke da arsenic da mercury. Dithiothreitol (DTT) yana da ƙamshi mai kaifi, wanda zai iya zama cutarwa ga lafiya saboda shaƙa da taɓa fata. Saboda haka, ya zama dole a kare shi yayin aiki, a sanya abin rufe fuska, safar hannu da tabarau, sannan a yi aiki a cikin murfin hayaki.