N-Ethylmaleemide CAS:128-53-0 Farashin Mai Masana'anta

Gyaran Sunadaran: NEM yana canza ragowar cysteine ​​a cikin sunadaran ta hanyar samar da adduct mai hadewa. Wannan gyare-gyaren na iya shafar ayyukan sunadaran, tsari, da hulɗa. Amsawar NEM tare da ƙungiyoyin sulfhydryl ya sa ya zama kayan aiki mai mahimmanci don nazarin aikin furotin da hanyoyin da suka dogara da thiol.

Hana Enzymes: Yawancin enzymes suna dogara ne akan ƙungiyoyin thiol don aikinsu na catalytic. Ta hanyar amsawa da ƙungiyoyin thiol, NEM na iya hana takamaiman enzymes. An yi amfani da wannan sosai don nazarin motsin enzyme, fahimtar hanyoyin enzymatic, da kuma tantance yiwuwar hana enzyme.

Nazarin Hulɗar Protein da Protein: Ana amfani da NEM sau da yawa don bincika hulɗar furotin da furotin. Ta hanyar toshe ragowar cysteine ​​mai amsawa, NEM yana hana samuwar haɗin disulfide tsakanin sunadaran da ke hulɗa, don haka yana wargaza hulɗar su. Wannan yana bawa masu bincike damar yin nazarin rawar da takamaiman cysteine ​​ke takawa a hulɗar furotin da furotin.

Nazarin Dokokin Redox: Ana amfani da NEM wajen nazarin hanyoyin daidaita redox a cikin furotin. Ta hanyar gyara cysteines masu amsawa, NEM na iya daidaita takamaiman yanayin furotin da hana iskar shaka ko rage ragowar cysteine ​​da ba a so. Wannan yana taimakawa wajen fahimtar rawar da siginar redox da hanyoyin kulawa masu dacewa da redox ke takawa.

Nazarin Haɗakar Sinadarai: Ana iya amfani da NEM a matsayin wakili mai haɗakar Sinadarai don daidaita hadaddun furotin ko hulɗar furotin da furotin. Ta hanyar gyara ragowar cysteine, NEM na iya ƙirƙirar haɗin gwiwa tsakanin sunadarai masu hulɗa, yana ba masu bincike damar bincika hadaddun furotin da tsarinsu. Wannan dabarar tana da amfani musamman a fannin ilimin halittar jiki da nazarin proteomics.

Gano Magani: Saboda iyawarsa ta yin mu'amala da ragowar cysteine, an yi amfani da NEM a binciken gano magunguna. Ana iya amfani da shi don gano da kuma fayyace abubuwan da za a iya yi wa magunguna ko kuma nazarin takamaiman magungunan da ake amfani da su wajen samar da sunadaran da ke da wadataccen cysteine.