Fluorescein Isothiocyante CAS:3326-32-7

Sifofin Hasken Haske: FITC tana da yawan fitowar haske mai haske kuma tana fitar da haske mai haske kore bayan an motsa ta a kusan 488 nm. Sifofin haskenta sun sa ta dace da gano ta ta amfani da dabarun daukar hoto daban-daban, kamar su na'urar daukar haske ta haske, na'urar daukar haske ta ruwa, da kuma na'urar daukar haske ta haske.

Haɗin Covalent: FITC ya ƙunshi ƙungiyar isothiocyanate wadda ke ba ta damar haɗawa da ƙungiyoyin amino da ke cikin furotin, ƙwayoyin rigakafi, da sauran ƙwayoyin halitta. Wannan haɗin covalent yana tabbatar da daidaito da takamaiman lakabi na ƙwayoyin da aka yi niyya, wanda ke ba da damar gano su da kuma ganin su.

Immunofluorescence: Ana amfani da ƙwayoyin rigakafi masu lakabi da FITC a gwaje-gwajen immunofluorescence don gano takamaiman antigens ko sunadarai a cikin ƙwayoyin halitta da kyallen takarda. Hasken kore mai haske da FITC ke fitarwa yana bawa masu bincike damar gano da kuma hango yadda ƙwayoyin da aka yi niyya ke yaɗuwa a cikin samfuran halittu.

Tsarin kwararar ƙwayoyin cuta: Ana amfani da ƙwayoyin rigakafi masu lakabi da FITC ko wasu ƙwayoyin halitta masu haɗuwa da fluorochrome don tantancewa da kuma rarraba ƙwayoyin halitta bisa ga alamun saman su ko abubuwan da ke cikin ƙwayoyin halitta. FITC ya dace da laser 488 nm da ake amfani da shi a cikin kayan aikin kwararar ƙwayoyin cuta.

Nazarin hulɗar furotin: Ana iya amfani da FITC a cikin nazarin hulɗar furotin da furotin don tantance haɗin kai da hulɗar sunadarai. Ta hanyar sanya wa takamaiman furotin lakabi da FITC, masu bincike za su iya yin nazarin hulɗar su ta amfani da dabaru kamar canja wurin kuzarin fluorescence resonance (FRET) ko polarization na fluorescence.

Bin diddigin ƙwayoyin halitta: Ana iya amfani da FITC don bin diddigin da kuma bin diddigin motsin ƙwayoyin halitta a cikin ƙwayoyin halitta ko kyallen halitta. Ta hanyar sanya wa ƙwayoyin halitta masu sha'awa suna da FITC, masu bincike za su iya nazarin inda suke, yanayinsu, da kuma yanayin safarar su a ainihin lokaci ta amfani da na'urar hangen nesa ta fluorescence.

Aikace-aikacen Ganewa: Ana amfani da na'urori masu auna ƙwayoyin cuta ko ƙwayoyin cuta masu lakabi da FITC a cikin ganewar asali don aikace-aikace daban-daban, gami da gano takamaiman ƙwayoyin cuta, magungunan cutar, ko abubuwan da ke nuna alamun cutar a cikin samfuran marasa lafiya. Waɗannan na'urorin suna ba da ganewar asali mai sauƙi da takamaiman, wanda ke ba da damar ganowa cikin sauri da daidaito.