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N, N-Bis (2-hydroxyethyl) -2-aminoethanesulfonic acid sodium gishiri CAS: 66992-27-6
N, N-Bis (2-hydroxyethyl) -2-aminoethanesulfonic acid sodium gishiri, wanda kuma aka sani da HEPES sodium gishiri, wani sinadaran fili da aka saba amfani da shi azaman pH buffering wakili a cikin nazarin halittu da kuma sinadaran dakunan gwaje-gwaje.Yana taimakawa wajen kiyaye tsayayyen pH, yana mai da shi manufa don aikace-aikace kamar al'adun tantanin halitta, ƙididdigar enzyme, nazarin furotin, electrophoresis, da ƙirar magunguna.HEPES sodium gishiri yana tabbatar da mafi kyawun yanayi don hanyoyin nazarin halittu kuma yana haɓaka daidaito da amincin sakamakon gwaji.
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S-Butyrylthiocholine iodide CAS: 1866-16-6
S-Butyrylthiocholine iodide wani sinadari ne da aka saba amfani dashi a cikin nazarin halittu da kuma enzymatic.Abu ne mai mahimmanci ga butyrylcholinesterase enzyme (BChE) kuma ana amfani dashi don auna ayyukansa.
Lokacin da S-Butyrylthiocholine iodide ya zama hydrolyzed ta BhE, yana samar da thiocholine da butyric acid a matsayin samfurori.Za a iya auna sakin thiocholine ta amfani da spectrophotometric ko fluorometric assay, yana ba da damar ƙididdige ayyukan BchE.
Ana amfani da S-Butyrylthiocholine iodide sau da yawa a cikin asibiti da saitunan bincike don kimanta ayyukan BhE a cikin samfurori irin su plasma jini ko kyallen takarda.Ana iya amfani da shi don tantance aikin enzymatic na BhE da rawar da zai iya takawa a cikin matakai daban-daban na nazarin halittu, da kuma a cikin ganewar asali da kuma lura da wasu yanayin kiwon lafiya.
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ABTS (2,2'-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) dimmonium gishiri) CAS: 30931-67-0
Diammonium 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonate), sau da yawa ake magana a kai da ABTS, wani abu ne da ake amfani da shi na chromogenic da aka saba amfani da shi a cikin ƙididdigar ƙwayoyin cuta, musamman a fagen ilimin enzymology.Wani fili ne na roba wanda ake amfani dashi don auna ayyukan enzymes daban-daban, ciki har da peroxidases da oxidases.
ABTS ba shi da launi a cikin nau'in oxidized amma ya juya blue-kore lokacin da oxidized ta hanyar enzyme a gaban hydrogen peroxide ko oxygen oxygen.Wannan canjin launi yana faruwa ne saboda samuwar radical cation, wanda ke ɗaukar haske a cikin bakan da ake gani.
Halin da ke tsakanin ABTS da enzyme yana samar da samfur mai launi wanda za'a iya auna ta hanyar hoto.Ƙarfin launi yana daidai da aikin enzymatic kai tsaye, yana ba masu bincike damar kimanta kinetics na enzyme ƙididdiga, hanawa enzyme, ko hulɗar enzyme-substrate.
ABTS yana da fa'idodi da yawa na aikace-aikace a fannoni daban-daban, gami da bincike na asibiti, binciken magunguna, da kimiyyar abinci.Yana da matukar kulawa kuma yana ba da kewayon ƙarfi mai faɗi, yana mai da shi mashahurin zaɓi don ƙididdigar ƙwayoyin halitta da yawa.
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4-NITROPHENYLΑ-D-MALTOHEXAOSIDE CAS:74173-30-1
4-Nitrophenyl α-D-maltohexaoside wani fili ne na rukunin haɗin gwiwar α-glycosidic.Ya fito ne daga maltose, wanda shine disaccharide wanda ya ƙunshi raka'a glucose guda biyu.A cikin wannan fili, ƙungiyar hydroxyl na rukunin glucose na farko an maye gurbinsu da wani nau'in nitrophenyl.
Ana amfani da wannan fili a matsayin maƙalli a cikin ƙididdigar enzymatic don nazarin ayyukan enzymes daban-daban, musamman waɗanda ke cikin metabolism na carbohydrate.Ƙungiyar nitrophenyl tana ba da damar ganowa cikin sauƙi da ƙididdige halayen enzymatic ta hanyar auna abin sha ko kyalli na samfurin da aka raba.
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PIPES CAS: 5625-37-6 Farashin Mai ƙira
PIPES (piperazine-1,4-bisethanesulfonic acid) wani fili ne na buffering na zwitterionic wanda aka saba amfani dashi a cikin binciken nazarin halittu da kwayoyin halitta.Yana da tasiri mai mahimmanci na pH tare da babban ƙarfin don kiyaye yanayin pH a cikin kewayon pH na 6.1 zuwa 7.5.PIPES yana da ƙaramin tsangwama tare da kwayoyin halitta kuma ya dace da ƙididdigar dogaro da zafin jiki.Ana amfani da shi sau da yawa a cikin fasahar gel electrophoresis da ƙirar magunguna a matsayin wakili mai ƙarfafawa.Gabaɗaya, PIPES abu ne mai dacewa kuma ana amfani da shi sosai a cikin saitunan gwaji daban-daban.
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3,3′,5,5′-Tetramethylbenzidine CAS:207738-08-7
3,3′,5,5′-Tetramethylbenzidine, wanda kuma aka sani da TMB, wani sinadari ne da aka saba amfani dashi azaman chromogenic substrate a cikin gwajin immunosorbent mai alaƙa da enzyme (ELISA) da sauran ƙididdigar ƙwayoyin cuta.Ana amfani da shi sau da yawa don ganowa da ƙididdige kasancewar enzymes irin su horseradish peroxidase (HRP) a cikin samfuran halitta daban-daban.TMB yana fuskantar canjin launi daga mara launi zuwa shuɗi a gaban waɗannan enzymes.Daga baya, ana iya dakatar da martani ta ƙara acid wanda ke canza launin shuɗi zuwa launin rawaya na ƙarshe.Ƙarfin launin rawaya yana daidai da yawan adadin enzyme, yana ba da izinin ƙididdigewa.
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APS-5 CAS: 193884-53-6 Farashin Mai ƙira
(4-Chlorophenyl)thio-methanol 1-(dihydrogen phosphate) gishiri disodium (1:2) wani sinadari ne wanda ke cikin nau'in abubuwan da aka samo acridine.Ya ƙunshi tsarin zobe na 10-methylacridine tare da ƙungiyar thioether da aka haɗe zuwa matsayi na 4-chlorophenyl.Har ila yau, mahallin ya ƙunshi ƙungiyar methanol da ƙungiyoyin phosphate guda biyu waɗanda ions sodium suka rabu da su.
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5-Bromo-4-chloro-3-indolyl phosphate disodium gishiri CAS: 102185-33-1
5-Bromo-4-chloro-3-indolyl phosphate disodium gishiri (BCIP) wani sinadari ne da aka saba amfani da shi a cikin ilimin halitta da aikace-aikacen biochemistry.Yana da chromogenic substrate ga alkaline phosphatase enzymes.
Ana amfani da BCIP sau da yawa tare da nitroblue tetrazolium (NBT) a matsayin abin da ake amfani da shi don gano ayyukan alkaline phosphatase.Lokacin da aka cire BCIP ta hanyar alkaline phosphatase, nau'in hazo mai shuɗi, yana ba da damar hangen nesa na kasancewar enzyme ko aiki.
Wannan fili yana da amfani musamman a aikace-aikace kamar immunohistochemistry, a cikin yanayin haɓakawa, da ƙididdigar immunosorbent mai alaƙa da enzyme (ELISAs) don gano kasancewar ko gano takamaiman ƙwayoyin halitta ko ƙwayoyin nucleic.Hazo mai shuɗi wanda BCIP ya kirkira yana ba da siginar bayyane wanda ke taimakawa wajen ganowa da kuma nazarin ƙwayoyin da aka yi niyya a cikin samfuran gwaji.
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Sodium Gishiri Gishiri CAS: 139-41-3 Manufacturer Price
N, N-Bis (2-hydroxyethyl) glycine sodium gishiri wani sinadari ne da aka yi amfani da shi azaman wakili na buffering a cikin aikace-aikace daban-daban na biochemical da biophysical.Yana taimakawa daidaita matakin pH a cikin yanayin gwaji, yana mai da shi amfani a cikin nazarin enzyme, binciken furotin, al'adun tantanin halitta, da dabarun gogewa na Yamma.
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4-Aminophthalhydrazide AMPPD CAS: 3682-14-2
4-Aminophthalhydrazide, kuma aka sani da 4-APhH, wani sinadari ne tare da tsarin kwayoyin C8H8N2O.Yana cikin nau'in mahadi na hydrazide kuma an samo shi daga phthalic acid.
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N-Acetyl-L-cysteine CAS: 616-91-1
N-Acetyl-L-cysteine (NAC) wani nau'i ne na amino acid cysteine da aka gyara.Yana ba da tushen cysteine kuma ana iya jujjuya shi cikin sauri zuwa cikin glutathione tripeptide, mai ƙarfi antioxidant a cikin jiki.An san NAC don maganin antioxidant da kaddarorin mucolytic, yana sa ya zama mai amfani a aikace-aikacen lafiya daban-daban.
A matsayin antioxidant, NAC yana taimakawa kare sel daga lalacewa ta hanyar radicals kyauta, nau'in oxygen mai amsawa, da gubobi.Hakanan yana goyan bayan haɗakar glutathione, wanda ke taka muhimmiyar rawa a cikin ayyukan detoxification na jiki da kiyaye tsarin garkuwar jiki mai kyau.
An yi nazarin NAC don yuwuwar fa'idodinta a cikin lafiyar numfashi, musamman ga mutanen da ke da yanayi kamar mashako na yau da kullun, COPD, da cystic fibrosis.An fi amfani da shi azaman abin tsinkaya don taimakawa bakin ciki da sassauta gamsai, yana sauƙaƙa share hanyoyin iska.
Bugu da ƙari kuma, NAC ta nuna alƙawarin tallafawa lafiyar hanta ta hanyar taimakawa wajen kawar da abubuwa masu guba, irin su acetaminophen, mai maganin ciwo na kowa.Hakanan yana iya samun tasirin kariya daga lalacewar hanta ta hanyar shan barasa.
Baya ga kaddarorin tallafin antioxidant da na numfashi, an bincika NAC don yuwuwar fa'idodinta a cikin lafiyar hankali.Wasu bincike sun nuna cewa yana iya samun tasiri mai kyau a kan matsalolin yanayi, irin su baƙin ciki da damuwa mai tsanani (OCD).
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Acetylthiocholine iodide CAS: 1866-15-5
Acetylthiocholine iodide wani sinadari ne na sinadari wanda aka saba amfani dashi azaman maƙasudi a ƙididdigar enzyme don auna ayyukan enzyme acetylcholinesterase (AChE).AChE wani enzyme ne wanda ke lalata neurotransmitter acetylcholine, mataki mai mahimmanci na kawo karshen watsa siginar tsakanin kwayoyin jijiya.
Lokacin da AChE ke aiki da acetylthiocholine iodide, an cire rukunin acetyl, wanda ya haifar da samuwar thiocholine da acetate ions.Thiocholine sai ya amsa tare da reagent mara launi da ake kira DTNB (5,5'-dithiobis(2-nitrobenzoic acid)) don samar da fili mai launin rawaya mai suna 5-thio-2-nitrobenzoate, wanda za'a iya auna shi ta hanyar spectrophotometrically.Matsakaicin ci gaban launi yana daidai da aikin AChE a cikin samfurin.
