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5-Bromo-4-chloro-3-indolyl phosphate disodium gishiri CAS:102185-33-1 Hoton da aka Fito
  • 5-Bromo-4-chloro-3-indolyl phosphate disodium gishiri CAS:102185-33-1

5-Bromo-4-chloro-3-indolyl phosphate disodium gishiri CAS:102185-33-1

5-Bromo-4-chloro-3-indolyl phosphate disodium gishiri (BCIP) wani sinadari ne da ake amfani da shi a fannin ilmin kwayoyin halitta da aikace-aikacen biochemistry. Yana da sinadarin chromogenic don enzymes na alkaline phosphatase.

Sau da yawa ana amfani da BCIP tare da nitroblue tetrazolium (NBT) a matsayin wani abu da ake amfani da shi wajen gano aikin alkaline phosphatase. Lokacin da alkaline phosphatase ya cire BCIP, sai wani abu mai kama da shuɗi ya bayyana, wanda hakan ke ba da damar ganin kasancewar ko aikin enzyme.

Wannan sinadari yana da amfani musamman a aikace-aikace kamar immunohistochemistry, in situ hybridization, da kuma enzyme-linked immunosorbent assays (ELISAs) don gano kasancewar ko wurin da takamaiman biomolecules ko nucleic acid suke. Shuɗin shudi da BCIP ta samar yana ba da siginar da za a iya gani wanda ke taimakawa wajen gano da kuma nazarin ƙwayoyin da aka yi niyya a cikin samfuran gwaji.

 


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Aikace-aikace da Tasiri

Immunohistochemistry (IHC): Sau da yawa ana amfani da BCIP a gwaje-gwajen IHC don ganin kasancewar ko gano takamaiman antigens a cikin samfuran nama. Ruwan shuɗi da BCIP ke samarwa yana ba da damar gano wuraren antigenic kuma ana iya amfani da shi don nazarin inganci ko rabin adadi.

In situ hybridization (ISH): Ana amfani da BCIP a cikin dabarun ISH don gano da kuma gano takamaiman jerin nucleic acid, kamar mRNA ko DNA. Ruwan shuɗi da BCIP ya samar yana nuna kasancewar jerin abubuwan da aka nufa kuma yana taimakawa wajen ganin yadda ake rarraba su a cikin ƙwayoyin halitta ko kyallen takarda.

Gwaje-gwajen immunosorbent da ke da alaƙa da enzyme (ELISAs): A wasu lokutan ana amfani da BCIP a cikin ELISAs don gano kasancewar ko yawan ƙwayoyin halitta na musamman, kamar sunadarai ko ƙwayoyin rigakafi. Ta hanyar haɗa BCIP da alamar enzyme mai dacewa da amfani da alkaline phosphatase don haɓaka amsawar, launin shuɗin da aka samar na iya nuna kasancewar da yawan ƙwayoyin halitta masu sha'awa.

Shirya Samfurin:

6892-68-8-3

Ƙarin Bayani:

Tsarin aiki C8H7BrClNNaO4P
Gwaji 99%
Bayyanar Foda fari
Lambar CAS 102185-33-1
shiryawa Ƙarami da girma
Rayuwar shiryayye Shekaru 2
Ajiya A adana a wuri mai sanyi da bushewa
Takardar shaida ISO.

 

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