Ethidium Bromide CAS:1239-45-8

Ethidium bromide (EtBr) wani nau'in rini ne mai haske wanda ake amfani da shi a fannin ilmin kwayoyin halitta da kuma ilmin sunadarai don ganin nucleic acid, musamman DNA, a cikin electrophoresis na gel agarose. Yana da babban alaƙa da DNA kuma, idan aka fallasa shi ga hasken ultraviolet (UV), yana haskakawa kuma yana fitar da launin ja-orange. Wannan yana bawa masu bincike damar gano da kuma yin nazarin gutsuttsuran DNA da aka raba ta hanyar girma a cikin matrix na gel cikin sauƙi.

EtBr yana haɗuwa tsakanin nau'ikan tushen DNA da RNA, wanda ke haifar da canji a cikin halayen haskensa. Yawanci ana ƙara shi a cikin gels na agarose ko a haɗa shi da samfuran DNA kafin electrophoresis. Lokacin da aka fallasa gel ɗin ga hasken UV, madannin DNA da ke ɗauke da EtBr suna bayyana a matsayin madannin orange mai haske a kan bango mai duhu, wanda hakan ke sauƙaƙa ganin da kuma nazarin gutsuttsuran DNA.

Yi oda ko Nemi Farashi

Cikakken Bayani game da Samfurin

Alamun Samfura

Aikace-aikace da Tasiri

Nuna DNA/RNA: Ana amfani da EtBr sosai don ganin DNA da RNA a cikin electrophoresis na gel na agarose. Yana haɗuwa tsakanin nau'ikan tushe na ƙwayoyin nucleic acid, yana sa su yi haske lokacin da aka fallasa su ga hasken UV. Wannan yana bawa masu bincike damar hangowa da kuma nazarin rabuwar gutsuttsuran DNA ko RNA bisa ga girmansu ko jerinsu.

Tabon DNA: Ana iya amfani da EtBr don yin tabon DNA a cikin hanyoyin gwaji daban-daban. Ana iya ƙara shi a cikin samfuran DNA, kamar plasmids masu tsabta ko samfuran PCR, don tabbatar da kasancewarsu da yawansu. Sannan ana iya ganin DNA mai tabon ta amfani da hasken UV ko tsarin ɗaukar hoto na gel.

Ƙididdige DNA: Ana iya amfani da EtBr don ƙididdige yawan DNA. Ta hanyar kwatanta ƙarfin madaurin DNA mai launi na EtBr a cikin gel na agarose tare da sanannun ma'aunin yawan DNA, za a iya tantance kimanin adadin DNA a cikin samfurin.

Na'urar Duba Hasken Haske: Ana iya amfani da EtBr a cikin na'urar duba haske don yin tabo da kuma ganin DNA a cikin ƙwayoyin halitta ko kyallen takarda. Yana ba masu bincike damar yin nazarin wurin DNA da kuma yadda yake aiki a cikin ƙwayoyin halitta ko kuma su lura da rarrabuwar DNA a cikin ƙwayoyin halitta.

Shirya Samfurin:

Ƙarin Bayani:

Tsarin aiki	C21H20BrN3
Gwaji	99%
Bayyanar	Foda ja zuwa launin shunayya mai duhu
Lambar CAS	1239-45-8
shiryawa	Ƙarami da girma
Rayuwar shiryayye	Shekaru 2
Ajiya	A adana a wuri mai sanyi da bushewa
Takardar shaida	ISO.