Disodium 4-nitrophenylphosphate CAS:4264-83-9

Babban tasirin da amfani da disodium 4-nitrophenylphosphate shine amfani da shi azaman substrate na chromogenic don gwajin enzyme na alkaline phosphatase. Lokacin da aka haɗa wannan mahaɗin da alkaline phosphatase, enzyme ɗin yana raba rukunin phosphate daga disodium 4-nitrophenylphosphate, wanda ke haifar da samuwar 4-nitrophenol.

4-nitrophenol da aka samar wani abu ne mai launin rawaya wanda za a iya gano shi ta hanyar spectrophotometric a 405 nm. Ana amfani da wannan canjin launi don auna aikin alkaline phosphatase a cikin samfura, kamar ruwan halittu ko lysates na tantanin halitta.

Amfani da disodium 4-nitrophenylphosphate a cikin gwaje-gwajen enzyme yana ba da hanya mai sauƙi don auna aikin alkaline phosphatase. Wannan enzyme yana da hannu a cikin hanyoyi daban-daban na halittu kuma ana amfani da shi azaman alamar bambance-bambancen nama, aikin hanta, da metabolism na ƙashi.

Bugu da ƙari, ana iya amfani da disodium 4-nitrophenylphosphate a matsayin madadin sauran phosphatases, kamar acid phosphatase ko furotin phosphatases, ya danganta da tsarin gwaji da burin bincike.