DEPC CAS:1609-47-8 Farashin Mai Masana'anta

Diethyl pyrocarbonate (DEPC) wani sinadari ne da ake amfani da shi a dakunan gwaje-gwajen ilmin halitta na kwayoyin halitta. Ruwa ne mai haske kuma mara launi wanda ke da matukar tasiri ga mahaɗan nucleophilic kamar amines da thiols. Ana amfani da DEPC musamman don dakatar da RNases (ribonucleases), enzymes waɗanda zasu iya lalata RNA, ta hanyar gyara wuraren aikinsu. Yana cimma wannan ta hanyar amsawa da ragowar histidine a cikin enzyme, waɗanda suke da mahimmanci ga aikinsa. Wannan ya sanya DEPC kayan aiki mai mahimmanci a cikin gwaje-gwajen da suka shafi RNA kuma yana tabbatar da kiyaye amincin RNA. Baya ga kaddarorin hana RNase, DEPC kuma yana iya gyara wasu kwayoyin halitta, kamar sunadarai da DNA, saboda amsawarsa da ƙungiyoyin nucleophilic. Ya kamata a yi taka tsantsan lokacin da ake sarrafa DEPC, saboda yana da guba kuma yana iya canzawa.

Yi oda ko Nemi Farashi

Cikakken Bayani game da Samfurin

Alamun Samfura

Aikace-aikace da Tasiri

Rashin Kunna RNases: Babban manufar DEPC ita ce dakatar da RNases, waɗanda enzymes ne waɗanda za su iya lalata RNA. DEPC tana amsawa da ragowar histidine a cikin RNases, tana gyara wuraren aiki kuma tana sa su zama marasa aiki. Wannan yana tabbatar da kiyaye amincin RNA yayin gwaje-gwaje kamar ware RNA, kwafi na baya, da kuma jerin RNA.

Kiyaye samfuran RNA: Sau da yawa ana amfani da maganin DEPC ga kayan aikin dakin gwaje-gwaje, kamar bututun microcentrifuge, tips na pipette, da ruwan da ake amfani da shi a gwaje-gwajen RNA. Wannan yana taimakawa wajen hana duk wani sauran RNAse da zai iya kasancewa da kuma hana lalacewar RNA yayin sarrafa samfura da adanawa.

Shirye-shiryen reagent marasa RNAase: Ana iya amfani da DEPC don magance mafita ko reagent waɗanda ke buƙatar rashin RNAase, kamar su buffers, maganin Tris-EDTA (TE), ko ruwa da ake amfani da shi a gwaje-gwajen ilmin halitta na kwayoyin halitta. Waɗannan mafita da aka yi wa magani za a iya amfani da su lafiya don rage lalacewar RNA.

Gyaran DNA: DEPC kuma tana iya yin aiki tare da ƙungiyoyin amino a cikin ƙwayoyin DNA, wanda ke haifar da canjin carbethylated na tushen DNA. Ana iya amfani da wannan a cikin takamaiman aikace-aikace, kamar halayen jerin DNA ko gyaran DNA don nazarin haɗin gwiwa.

Shirya Samfurin:

Ƙarin Bayani:

Tsarin aiki	C6H10O5
Gwaji	99%
Bayyanar	Ruwa mara launi
Lambar CAS	1609-47-8
shiryawa	Ƙarami da girma
Rayuwar shiryayye	Shekaru 2
Ajiya	A adana a wuri mai sanyi da bushewa
Takardar shaida	ISO.