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BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine gishiri CAS:6578-06-9 Hoton da aka Fito
  • BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine gishiri CAS:6578-06-9

BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine gishiri CAS:6578-06-9

5-Bromo-4-chloro-3-indolyl phosphate gishirin p-toluidine wani sinadari ne da ake amfani da shi a fannin ilmin kwayoyin halitta da binciken sinadarai. Sau da yawa ana amfani da shi a cikin gwaje-gwajen immunosorbent na enzyme-linked (ELISA) da dabarun lakabin furotin.

Wannan sinadari wani sinadari ne da ake amfani da shi wajen samar da sinadarin alkaline phosphatase, wani sinadari ne da ake amfani da shi sosai a fannin ilmin kwayoyin halitta don gano kasancewar takamaiman kwayoyin halitta ko sunadaran ta hanyar halayen launi ko na chemiluminescent.

Idan akwai sinadarin alkaline phosphatase, yana raba rukunin phosphate daga gishirin p-toluidine 5-Bromo-4-chloro-3-indolyl phosphate, wanda ke haifar da samuwar launin shuɗi ko shunayya. Ana iya gano wannan canjin launi cikin sauƙi kuma a auna shi, wanda ke ba da damar auna ayyukan enzyme ko kasancewar ƙwayoyin da aka yi niyya.


Cikakken Bayani game da Samfurin

Alamun Samfura

Aikace-aikace da Tasiri

Western blotting: Ana amfani da BCIP don gano da kuma ganin sunadaran da aka canja zuwa membranes bayan gel electrophoresis. Ana amfani da magungunan rigakafi na biyu da aka haɗa da alkaline phosphatase don ɗaurewa da manyan ƙwayoyin rigakafi, kuma bayan haka tare da BCIP yana haifar da ci gaban wani abu mai launin shuɗi a wurin da aka haɗa ƙwayoyin furotin.

Immunohistochemistry: Ana amfani da BCIP a dabarun yin tabo na immunohistochemical don gano kasancewar takamaiman sunadarai a cikin kyallen takarda. Ana amfani da magungunan rigakafi na biyu masu haɗin alkaline phosphatase don ɗaurewa da manyan ƙwayoyin rigakafi, kuma maganin BCIP yana haifar da launin shuɗi a gaban enzyme, yana nuna wurin furotin a cikin samfurin kyallen.

Haɗakarwa a cikin yanayi: Ana amfani da BCIP don gano jerin ƙwayoyin nucleic acid a cikin kyallen takarda ko ƙwayoyin halitta masu tsayayye. Ana yi wa na'urorin haɗakarwa lakabi da alkaline phosphatase, kuma bayan ƙara BCIP, kasancewar mRNA ko DNA da aka yi niyya ana nuna shi ta hanyar haɓakar launin shuɗi.

Shirya Samfurin:

试剂包装2

Ƙarin Bayani:

Tsarin aiki C8H6BrClNO4P.C7H9N
Gwaji 99%
Bayyanar Foda fari
Lambar CAS 6578-06-9
shiryawa Ƙarami da girma
Rayuwar shiryayye Shekaru 2
Ajiya A adana a wuri mai sanyi da bushewa
Takardar shaida ISO.

 

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