BCIP Na) 5-Bromo-4-chloro-3-indolyl phosphate disodium gishiri CAS:102185-33-1

Tasiri:

BCIP wani sinadari ne mara launi wanda ake mayar da shi zuwa ruwan shuɗi-shuɗi bayan aikin alkaline phosphatase. Wannan canjin launi yana ba da damar gano gani da kuma ƙididdige ayyukan enzyme.

Aikace-aikace:

Gwaje-gwajen Enzyme: Ana amfani da BCIP a gwaje-gwajen sinadarai daban-daban don auna aikin alkaline phosphatase. Wannan ya haɗa da gano ayyukan phosphatase a cikin ƙwayoyin halitta, enzymes masu tsabta, ko gwaje-gwajen in vitro.

Immunohistochemistry: Ana iya amfani da BCIP don ganin ƙwayoyin rigakafi masu lakabin alkaline phosphatase a sassan nama ko a saman tantanin halitta. Sau da yawa ana amfani da wannan dabarar a cikin ka'idojin rage tasirin rigakafi.

Dabaru na Blotting: Ana amfani da BCIP a cikin Western blotting, Southern blotting, da Northern blotting don gano takamaiman sunadarai ko nucleic acid waɗanda aka yiwa lakabi da alkaline phosphatase-conjugated probes.

Haɗakar kwayoyin halitta a wuri: Ana iya amfani da BCIP don hango yanayin bayyanar kwayoyin halitta a cikin kyallen takarda ko ƙwayoyin halitta ta hanyar hanyoyin haɗakar kwayoyin halitta a wuri.

Gwaje-gwajen Ganewa: Ana amfani da BCIP a matsayin wani abu mai kama da sinadarai a cikin kayan bincike daban-daban don gano sinadarin alkaline phosphatase. Ana amfani da waɗannan kayan a dakunan gwaje-gwaje na asibiti don gano cututtuka da kuma sa ido.