ABTS (2,2'-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) dimmonium gishiri) CAS: 30931-67-0

Diammonium 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonate), sau da yawa ake magana a kai da ABTS, wani abu ne da ake amfani da shi na chromogenic da aka saba amfani da shi a cikin ƙididdigar ƙwayoyin cuta, musamman a fagen ilimin enzymology.Wani fili ne na roba wanda ake amfani dashi don auna ayyukan enzymes daban-daban, ciki har da peroxidases da oxidases.

ABTS ba shi da launi a cikin nau'in oxidized amma ya juya blue-kore lokacin da oxidized ta hanyar enzyme a gaban hydrogen peroxide ko oxygen oxygen.Wannan canjin launi yana faruwa ne saboda samuwar radical cation, wanda ke ɗaukar haske a cikin bakan da ake gani.

Halin da ke tsakanin ABTS da enzyme yana samar da samfur mai launi wanda za'a iya auna ta hanyar hoto.Ƙarfin launi yana daidai da aikin enzymatic kai tsaye, yana ba masu bincike damar kimanta kinetics na enzyme ƙididdiga, hanawa enzyme, ko hulɗar enzyme-substrate.

ABTS yana da fa'idodi da yawa na aikace-aikace a fannoni daban-daban, gami da bincike na asibiti, binciken magunguna, da kimiyyar abinci.Yana da matukar kulawa kuma yana ba da kewayon ƙarfi mai faɗi, yana mai da shi mashahurin zaɓi don ƙididdigar ƙwayoyin halitta da yawa.

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Aikace-aikace da Tasiri

Enzymatic Assays: ABTS ana amfani dashi sosai don auna ayyukan enzymes kamar peroxidases da oxidases.Yana aiki azaman maɓalli don waɗannan enzymes, kuma ana iya ƙididdige ayyukansu ta hanyar auna ƙarfin samfurin da aka samar.

Takaddun Ƙarfin Ƙarfin Antioxidant: ABTS galibi ana aiki da shi a cikin gwajin ƙarfin ƙarfin antioxidant don tantance ikon abubuwa don lalata ko hana radicals kyauta.Samuwar launi a gaban wani maganin antioxidant yana nuni da iyawar sa mai tsattsauran ra'ayi.

Ƙididdigar Protein: Ana iya amfani da ABTS don tantance jimlar furotin a cikin samfurori na halitta.Halin ABTS tare da jan ƙarfe mai ɗaure sunadaran yana haifar da samuwar samfur mai launi wanda za'a iya ƙididdige shi.Wannan hanya an fi sani da bicinchoninic acid (BCA) assay.

Gano Drug: Ana amfani da ABTS a cikin manyan gwaje-gwajen gwaje-gwaje don kimanta ayyukan antioxidant na mahaɗan magungunan ƙwayoyi.Wannan yana ba masu bincike damar gano mahadi tare da tasirin maganin warkewa.

Masana'antar Abinci da Abin Sha: Ana amfani da ABTS a masana'antar abinci da abin sha don tantance ƙarfin antioxidant na samfuran abinci daban-daban, kamar 'ya'yan itatuwa, kayan marmari, da abubuwan sha.Yana taimakawa kimanta fa'idodin kiwon lafiya da kwanciyar hankali na waɗannan samfuran.

Kula da Muhalli: Ana iya amfani da ABTS don tantance jimillar ƙarfin maganin antioxidant na samfuran muhalli, yana taimakawa wajen kimanta matakan gurɓata yanayi da tasirin su akan muhalli.

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Ƙarin Bayani:

Abun ciki	Saukewa: C18H24N6O6S4
Assay	99%
Bayyanar	Koren foda
CAS No.	30931-67-0
Shiryawa	Karami da girma
Rayuwar Rayuwa	shekaru 2
Adana	Ajiye a wuri mai sanyi da bushewa
Takaddun shaida	ISO.