5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium gishiri CAS:18656-96-7

5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide Gishirin cyclohexylammonium, wanda aka fi sani da X-glucuronide, wani sinadari ne da ake amfani da shi a gwaje-gwajen ilmin kwayoyin halitta da kuma enzymology. Wani sinadari ne mai kama da chromogenic wanda ake amfani da shi wajen gano aikin enzyme beta-D-glucuronidase.

Wannan sinadarin ya ƙunshi rukunin indole da aka haɗa shi da wani sinadari na glucuronic acid ta hanyar haɗin glycosidic. Abubuwan da ke cikin bromo da chloro suna ba da kwanciyar hankali kuma suna haɓaka narkewar sinadarin.

Idan aka saka shi da samfurin da ke ɗauke da beta-D-glucuronidase, enzyme ɗin yana raba haɗin glycosidic, yana fitar da ɓangaren indole. Wannan amsawar tana haifar da samuwar shuɗin da ke fitowa ko canza launi, wanda ke ba da damar gano ayyukan enzyme cikin sauƙi ko kuma a bayyane.

Saboda takamaimansa ga beta-D-glucuronidase, ana amfani da X-glucuronide sosai a cikin dabarun ilmin kwayoyin halitta kamar gwajin canjin ƙwayoyin cuta da yisti, gwajin kwayoyin halitta mai rahoto, da kuma tabon histochemical. Yana samar da hanya mai sauƙi da sauƙi don gano kasancewar ko bayyanar beta-D-glucuronidase kuma galibi ana amfani da shi tare da wasu gwaje-gwajen kwayoyin halitta ko na biochemical.

Yi oda ko Nemi Farashi

Cikakken Bayani game da Samfurin

Alamun Samfura

Aikace-aikace da Tasiri

Tasirin gishirin cyclohexylammonium na 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide (X-glucuronide) shine ikonsa na samar da shudi mai haske ko canjin launi a gaban enzyme beta-D-glucuronidase. Wannan amsawar enzymatic tana faruwa ne lokacin da aka raba haɗin glycosidic tsakanin ƙungiyar indole da ɓangaren glucuronic acid ta hanyar beta-D-glucuronidase.

Amfani da X-glucuronide galibi yana cikin gwaje-gwajen ilmin kwayoyin halitta da kuma enzymology. Ana amfani da shi azaman substrate don gano ayyukan beta-D-glucuronidase. Wasu takamaiman aikace-aikace sun haɗa da:

Gwajin kwayoyin halitta na masu rahoto: Ana amfani da X-glucuronide sau da yawa a matsayin wani abu a cikin gwaje-gwajen kwayoyin halitta na masu rahoto inda ake amfani da beta-D-glucuronidase a matsayin enzyme mai ba da rahoto. Ta hanyar haɗa yankin mai haɓaka ko kuma rubuta jerin kwayoyin halitta masu sha'awa ga kwayar halittar beta-D-glucuronidase, masu bincike za su iya auna bayyanar ko aikin kwayar halitta ta hanyar sa ido kan samuwar shuɗin da ke fitowa ko canjin launi lokacin da aka ƙara X-glucuronide.

Gwaje-gwajen canza ƙwayoyin cuta da yisti: Ana iya amfani da X-glucuronide don gano nasarar canjin ƙwayoyin cuta ko yisti ta hanyar amfani da vector wanda ke ɗauke da kwayar halittar beta-D-glucuronidase. Kwayoyin da aka canza waɗanda ke bayyana enzyme na iya yin hydrolyze na X-glucuronide, yana haifar da canjin launi ko fashewar da ake gani.

Tabon Histochemical: Ana amfani da X-glucuronide a cikin dabarun tabon histochemical don hango kasancewar da rarrabawar ayyukan beta-D-glucuronidase a cikin kyallen takarda ko ƙwayoyin halitta. Wannan tabon yana ba da damar gano ƙwayoyin da ke bayyana enzyme.

Shirya Samfurin:

Ƙarin Bayani:

Tsarin aiki	C26H36BrClN2O7
Gwaji	99%
Bayyanar	Foda fari
Lambar CAS	18656-96-7
shiryawa	Ƙarami da girma
Rayuwar shiryayye	Shekaru 2
Ajiya	A adana a wuri mai sanyi da bushewa
Takardar shaida	ISO.