4-Nitrophenyl Beta-D-glucuronide CAS:10344-94-2

4-Nitrophenyl Beta-D-glucuronide wani sinadari ne da aka samar ta hanyar haɗa kwayar glucose zuwa ga rukunin nitrophenyl 4 ta hanyar haɗin glycosidic. Ana amfani da shi a matsayin substrate a cikin gwaje-gwajen enzymatic don gano kasancewar da aikin β-glucuronidase, wani enzyme da ke da hannu a cikin metabolism na magunguna daban-daban da xenobiotics a cikin dabbobi masu shayarwa. Lokacin da β-glucuronidase ya kasance, yana raba haɗin glycosidic tsakanin rukunin glucose da 4-nitrophenyl, wanda ke haifar da sakin 4-nitrophenol, wanda za'a iya gano shi ta hanyar spectrophotometric a 400-420 nm. Wannan amsawar enzymatic yana ba da ma'aunin adadi na aikin β-glucuronidase kuma galibi ana amfani da shi azaman kayan aiki a cikin gano magunguna, nazarin guba, da kuma binciken asibiti.

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Gano ayyukan β-glucuronidase: Ana amfani da 4-NPBG a matsayin wani abu mai kama da chromogenic substrate don tantance kasancewar da ayyukan β-glucuronidase a cikin samfuran halittu daban-daban. Enzyme ɗin yana raba haɗin glycosidic na 4-NPBG, yana samar da 4-nitrophenol, wanda za'a iya aunawa ta amfani da spectrophotometry.

Nazarin metabolism na magunguna: Tunda β-glucuronidase yana taka muhimmiyar rawa a cikin metabolism na magunguna da xenobiotics, ana iya amfani da 4-NPBG don tantance ayyukan wannan enzyme a cikin nazarin metabolism na magunguna. Yana taimakawa wajen fahimtar girman da motsin halayen glucuronidation, waɗanda suke da mahimmanci don kawar da magunguna da kuma samar da bioavailability.

Nazarin Toxicology: Wasu sinadarai masu guba za a iya narkewa su kuma a fitar da su ta hanyar glucuronide conjugates. Ta hanyar amfani da 4-NPBG a matsayin substrate, masu bincike za su iya bincika ayyukan β-glucuronidase a cikin kyallen takarda ko layukan tantanin halitta daban-daban don tantance yuwuwar guba ko mummunan tasirin mahadi.

Ganewar Asibiti: Ana iya amfani da auna aikin β-glucuronidase ta amfani da 4-NPBG a wuraren asibiti don gano wasu cututtuka ko yanayi. Matakan da ba su dace ba ko ayyukan β-glucuronidase na iya nuna wasu matsalolin kwayoyin halitta, rashin aikin hanta, ko ciwon daji.

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Ƙarin Bayani:

Tsarin aiki	C12H13NO9
Gwaji	99%
Bayyanar	Foda fari
Lambar CAS	10344-94-2
shiryawa	Ƙarami da girma
Rayuwar shiryayye	Shekaru 2
Ajiya	A adana a wuri mai sanyi da bushewa
Takardar shaida	ISO.