4-Nitrophenyl beta-D-galactpyranoside CAS: 200422-18-0

Tasiri: ONPG wani abu ne da aka yi amfani da shi musamman don gano gabanin da ayyukan enzyme β-galactosidase.Lokacin da β-galactosidase enzyme ya kasance kuma yana aiki, yana raba ONPG zuwa samfurori guda biyu: o-nitrophenol da kuma abin da aka samu na galactose.'Yancin o-nitrophenol yana haifar da canjin launin rawaya, wanda za'a iya auna ta amfani da spectrophotometer.

Aikace-aikace: ONPG yana da aikace-aikace da yawa a cikin ilimin halitta da bincike na biochemistry:

Ƙaddamar da aikin β-galactosidase: ONPG ana amfani dashi don aunawa da ƙididdige ayyukan β-galactosidase enzyme.Adadin samuwar o-nitrophenol, wanda ke daidai da aikin enzyme, ana iya auna shi ta hanyar spectrophotometrically.

Maganar Halitta da ƙa'ida: Ana amfani da ONPG sau da yawa a cikin gwaje-gwajen da suka shafi maganganun kwayoyin halitta da nazarin ƙa'ida.Ana amfani dashi akai-akai a cikin gwaje-gwajen furotin na fusion, kamar tsarin haɗakar lacZ da aka saba amfani da shi, don nazarin maganganun kwayoyin halitta a ƙarƙashin kulawar takamaiman masu tallatawa.Ayyukan beta-galactosidase da aka auna ta amfani da ONPG yana ba da haske game da matakin bayanin kwayoyin halitta.

Nunawa don ayyukan β-galactosidase: ONPG za a iya amfani dashi azaman hanyar nunawa mai launi a cikin fasahar DNA ta sake haɗawa don gano kasancewar da aiki na kwayar LacZ, wanda ke ɓoye β-galactosidase.Wannan hanyar tantancewa tana taimakawa wajen gano clones waɗanda ke ɗauke da kwayar halittar sha'awa.

Nazarin kinetics na Enzyme: ONPG kuma yana da amfani a cikin nazarin kinetics na β-galactosidase enzyme.Ta hanyar auna ƙimar amsawar enzyme-substrate a ma'auni daban-daban, yana yiwuwa a ƙayyade sigogin motsi kamar Michaelis-Menten akai-akai (Km) da matsakaicin ƙimar amsawa (Vmax).