4-Methylumbelliferyl-beta-D-glucopyranoside CAS: 18997-57-4

Tasirin 4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) shine don yin aiki azaman ma'auni na beta-glucosidase enzyme.Wannan enzyme yana lalata haɗin glucosidic na MUG, wanda ya haifar da sakin 4-Methylumbelliferone (4-MU) .Aikace-aikacen MUG shine da farko a fagen ilimin ƙwayoyin cuta, musamman don ganowa da gano ƙwayoyin cuta na beta-glucosidase.Ana yawan amfani da MUG wajen gano Escherichia coli (E. coli) a cikin ruwa da samfuran abinci.E. coli yana da enzyme beta-glucosidase, wanda zai iya hydrolyze MUG kuma ya samar da sigina mai kyalli a gaban hasken ultraviolet (UV). Abubuwan da ke da haske na 4-MU yana ba da damar ganowa da ƙididdigewa mai sauƙi.Lokacin da MUG substrate aka narkar da ruwa, 4-MU da aka samar yana fitar da haske mai shuɗi, yana sauƙaƙe gano ƙwayoyin cuta waɗanda ke da aikin beta-glucosidase.Ana amfani da wannan hanyar da yawa a cikin kulawa da muhalli da gwajin amincin abinci, saboda tana ba da hanya mai sauri da mahimmanci don gano gurɓataccen ƙwayar cuta. Baya ga aikace-aikacen sa a cikin ƙwayoyin cuta, ana iya amfani da MUG don yin nazarin ayyuka da hana beta-glucosidase a ciki. Biochemistry da bincike na kwayoyin halitta.Haskensa yana ba da damar ma'aunin kinetics na enzyme kuma ana iya amfani dashi don nunawa ga masu hanawa masu hanawa ko masu kunna ayyukan beta-glucosidase. Gabaɗaya, MUG wani fili ne mai mahimmanci wanda ke samun aikace-aikacen tartsatsi a cikin fagagen microbiology, biochemistry, da ilimin halittu don ganowa. na ayyukan beta-glucosidase da gano ƙwayoyin cuta waɗanda ke samar da wannan enzyme.