4-Amino-3-hydrazino-1,2,4-triazol-5-thiol CAS:28836-03-5

Tasirin 3,3'-Diaminobenzidine (DAB) shine samar da launin ruwan kasa lokacin da yake amsawa da enzymes na peroxidase. Wannan canjin launi yana ba da damar ganin antigens ko biomolecules masu sha'awar gwajin immunohistochemistry (IHC) da immunocytochemistry (ICC). Sau da yawa ana amfani da DAB a cikin binciken likitanci da aikace-aikacen bincike don gano da kuma gano takamaiman antigens ko sunadarai a cikin samfuran halittu. Ta hanyar ƙara DAB a matsayin substrate da amsawa daga baya tare da enzymes peroxidase, ana samar da ruwan kasa mai narkewa a wurin da antigens ɗin da aka nufa. Amfani da tabo na DAB yana da amfani musamman wajen nazarin cututtuka da yanayi daban-daban. Yana ba masu bincike da masana cututtuka damar hangowa da kuma nazarin rarrabawa, yalwa, da kuma gano takamaiman biomolecules a cikin kyallen takarda ko ƙwayoyin halitta. Wannan bayanin yana taimakawa wajen fahimtar hanyoyin cututtuka, nazarin hanyoyin ƙwayoyin halitta, da kuma gano yiwuwar gano ko magance matsalolin da za a iya fuskanta. Bugu da ƙari, ana iya amfani da fenti na DAB a cikin dabarun kwayoyin halitta daban-daban don bincika hulɗar furotin-protein, gano tsarin bayyanar furotin, tantance kasancewar takamaiman alamomi, da kuma kimanta tasirin hanyoyin magancewa. Saboda ingantaccen fenti mai ƙarfi, da kuma dacewarsa da tsarin ganowa daban-daban da dabarun microscopy, DAB ya zama wani abu mai amfani da chromogenic substrate a cikin gwaje-gwajen immunohistochemistry da immunocytochemistry.