3,3′,5,5′-Tetramethylbenzidine sulfate CAS:54827-18-8

ELISA: Ana amfani da shi sosai a matsayin wani abu da ake amfani da shi wajen samar da horseradish peroxidase (HRP) a gwaje-gwajen ELISA. A gaban kwayar halittar da aka yi niyya, kamar antigen ko antibody, enzyme HRP yana ƙarfafa oxidation na 3,3', 5,5'-Tetramethylbenzidine sulfate, wanda ke haifar da samuwar samfurin shuɗi. Ƙarfin launin yana daidai da yawan ƙwayar da aka yi niyya, wanda ke ba da damar yin nazarin adadi.

Gwaje-gwajen ayyukan enzyme: Ana iya amfani da 3,3',5,5'-Tetramethylbenzidine sulfate don auna aikin enzyme. Ta hanyar sanya samfurin enzyme tare da substrate, ana iya sa ido kan amsawar enzyme ta hanyar canjin launi. Babban aikin enzyme yana haifar da launin shuɗi mai ƙarfi, wanda ke ba da damar tantance motsin enzyme da nazarin hana shi.

Immunohistochemistry: Ana iya amfani da shi don yin tabo a cikin immunohistochemistry (IHC). Ana amfani da shi azaman chromogen don maganin rigakafi na farko ko na sakandare wanda aka yiwa lakabi da horseradish peroxidase, wanda ke haifar da samuwar wani wuri mai launin shuɗi a wurin hulɗar antigen da antibody.

Binciken sinadarai: 3,3',5,5'-Tetramethylbenzidine sulfate ya sami aikace-aikace a cikin nazarin bincike daban-daban na sinadarai, musamman a cikin gano da auna ayyukan enzymes, hulɗar furotin-protein, da hulɗar furotin-DNA.