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X-GAL CAS: 7240-90-6 Farashin Mai ƙira

5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) abu ne na yau da kullun na chromogenic da aka yi amfani da shi a cikin ilimin halitta da aikace-aikacen biochemistry.Ana amfani da shi sosai don gano ƙwayar lacZ, wanda ke ɓoye enzyme β-galactosidase.


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Aikace-aikace da Tasiri

Canjin Launi: X-Gal yawanci ba shi da launi amma, akan hydrolysis ta β-galactosidase, ya zama shuɗi.Wannan canjin launi yana ba da damar gano gani na gani da ƙididdige ayyukan β-galactosidase.

Ganewar LacZ Gene: Ana amfani da X-Gal don gano ƙwayoyin halitta ko abubuwan gina jiki waɗanda ke bayyana kwayar lacZ.Ana amfani da LacZ akai-akai azaman ɗan rahoto a cikin ilimin halitta don tantance maganganun kwayoyin halitta ko nazarin ayyukan masu tallata.

Nunin Mallaka: Ana amfani da X-Gal sau da yawa a gwajin gwajin ƙwayar cuta.LacZ-bayyanar da ƙwayoyin cuta suna bayyana shuɗi lokacin girma akan agar mai ɗauke da X-Gal, yana ba da sauƙin ganewa da zaɓin yankuna masu kyau na lacZ.

Binciken Fusion Gene: Hakanan ana amfani da X-Gal a cikin gwaje-gwajen fusion ɗin halittar.Lokacin da aka haɗu da ƙwayar da aka yi niyya da kwayar lacZ, tabon X-Gal na iya bayyana yanayin bayyanar furotin fusion a cikin tantanin halitta ko nama.

Ƙwararren Ƙwararrun Protein: Ana iya amfani da tabon X-Gal don bincika abubuwan gina jiki na subcellular.Ta hanyar haɗa furotin mai ban sha'awa ga jinsin lacZ, aikin β-galactosidase zai iya nuna inda furotin ke cikin tantanin halitta.

X-Gal Analogues: An ɓullo da gyare-gyaren nau'ikan X-Gal, irin su Bluo-Gal ko Red-Gal, don ba da izini ga madadin tsarin haɓaka launi.Waɗannan analogues suna ba da damar bambancewa tsakanin lacZ-tabbatacce da lacZ-korau sel ko kyallen takarda ta amfani da launi daban-daban.

Samfurin Samfura

7240-90-6-1
7240-90-6-2

Shirya samfur:

6892-68-8-3

Ƙarin Bayani:

Abun ciki Saukewa: C14H15BrClNO6
Assay 99%
Bayyanar Farin foda
CAS No. 7240-90-6
Shiryawa Karami da girma
Rayuwar Rayuwa shekaru 2
Adana Ajiye a wuri mai sanyi da bushewa
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