5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide wani fili ne da aka yi amfani da shi a cikin nazarin nazarin halittu daban-daban, musamman don ganowa da hangen nesa na ayyukan enzyme.Abu ne mai sauƙi wanda za'a iya sanya shi ta hanyar takamaiman enzymes, wanda ke haifar da sakin samfur mai launi ko mai kyalli.
Ana amfani da wannan fili da yawa a cikin gwaje-gwaje don gano kasancewar da ayyukan enzymes kamar beta-galactosidase da beta-glucuronidase.Wadannan enzymes sun raba acetyl da glucosaminide kungiyoyin daga substrate, haifar da samuwar blue ko kore chromophore.
Tsarin musamman na 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide yana ba da damar gano sauƙi da ƙididdige ayyukan enzyme.Amfani da shi a cikin fasahohin gwaji daban-daban, gami da histochemistry, immunohistochemistry, da ƙididdigar tushen tantanin halitta, ya ba da gudummawa ga kyakkyawar fahimtar ayyukan enzyme.