Akwai jarumai da dama da ba a waka a kusa da mu, wadanda ake ganin kamar talakawa ne, amma a gaskiya shiru suke ba mu gudunmawar da yawa.Proteinase K shine "jarumin da ba'a so" a cikin masana'antar bincikar kwayoyin halitta, kodayake idan aka kwatanta da "babba da karfi" a cikin masana'antar, proteinase K yana da ƙarancin maɓalli wanda mun daɗe da yin watsi da mahimmancinsa.Tare da barkewar sabuwar cutar kambi, buƙatun furotin K ya karu, kuma wadata a gida da waje yana da nisa bayan amfani, kuma kowa da kowa ya gane cewa furotin K yana da mahimmanci.
Menene amfanin proteinase K?
Proteinase K shine serine protease tare da aikin enzyme proteolytic kuma zai iya kula da aiki a cikin wurare masu yawa (pH (4-12.5), babban gishiri mai gishiri, babban zafin jiki na 70 ° C, da dai sauransu).Bugu da ƙari, aikin proteinase K ba a hana shi ta hanyar SDS, urea, EDTA, guanidine hydrochloride, guanidine isothiocyanate, da dai sauransu, kuma wani nau'i na kayan wankewa zai iya inganta aikin proteinase K. A cikin maganin likita (virus da ƙwayar cuta ta microbial disinfection). ), abinci (ƙasa nama), fata (laushin gashi), ruwan inabi (bayanin barasa), shirye-shiryen amino acid (ƙasassun gashin fuka-fukan), cirewar acid nucleic, in situ hybridization, da dai sauransu, proteinase K Akwai aikace-aikace.Mafi yawan aikace-aikacen da ake amfani da shi shine cirewar acid nucleic.
Proteinase K na iya enzymolyze kowane nau'in sunadaran da ke cikin samfurin, gami da waɗancan histones waɗanda ke daure sosai da acid nucleic, ta yadda za a iya sakin acid nucleic daga samfurin kuma a sake su a cikin tsantsa, sauƙaƙe mataki na gaba na hakar da tsarkakewa.A cikin gano kwayar nucleic acid, proteinase K yana ɗaya daga cikin mahimman abubuwan da ke cikin maganin samfurin ƙwayar cuta.Proteinase K na iya fashe kuma ya hana furotin gashi na kwayar cutar, wanda ya fi aminci yayin sufuri da matakin ganowa;Bugu da kari, proteinase K kuma na iya lalata RNase yana hana lalatawar kwayar cutar RNA kuma yana sauƙaƙe gano acid nucleic.
Shahararren furotin K
Ko a fagen binciken kimiyya ko kuma a fagen IVD, hako acid nucleic shine mafi mahimmancin gwaji, don haka proteinase K ya kasance mai mahimmancin rayuwa.Duk da haka, a baya, proteinase K ya kasance sananne sosai fiye da rawar da yake takawa.Babban ɓangare na wannan shine saboda wadata da alaƙar buƙata na proteinase K ya kasance karko sosai.Mutane kaɗan ne za su yi tunanin cewa samar da furotin K zai zama matsala.
Tare da barkewar sabuwar annoba ta kambi, buƙatar gwajin gwajin nucleic acid ya karu.Ya zuwa karshen watan Yuni na shekarar 2020, kasar Sin ta kammala gwaje-gwajen kambi kusan miliyan 90, kuma wannan adadi ya fi tayar da hankali a duniya.A cikin gwaje-gwajen hakar acid nucleic, yawan aiki na proteinase K shine kusan 50-200 μg/mL.Gabaɗaya, yana ɗaukar kusan 100 μg na proteinase K don cire samfurin nucleic acid.A cikin ainihin amfani, don haɓaka haɓakar haɓakar haɓakar acid nucleic, sau da yawa Proteinase K za a yi amfani da shi a cikin ƙarin adadin.Gano acid nucleic na sabon coronavirus ya kawo adadin furotin K bukatar.Asalin wadata da ma'aunin buƙatun furotin K ya karye cikin sauri, kuma proteinase K ya zama muhimmin kayan rigakafin annoba cikin dare.
Matsalolin samar da proteinase K
Ko da yake tare da ci gaban cutar, mahimmancin darajar proteinase K yana da daraja ga mutane, abin kunya ne cewa saboda ƙananan maɓalli na furotin K, ƙananan kamfanoni na cikin gida sun shiga cikin samar da furotin K. Lokacin da mutane suka shiga. Ana so a kafa samar da proteinase K A lokacin aikin samarwa, an gano cewa proteinase K wani sunadari ne na musamman.Yana da matuƙar ƙalubale don faɗaɗa ƙarfin samar da proteinase K a cikin ɗan gajeren lokaci.
Samar da babban sikelin proteinase K yana fuskantar matsaloli masu zuwa
1. Ƙananan magana
Proteinase K na iya ƙasƙantar da mafi yawan sunadaran ba musamman kuma ya haifar da mummunar guba ga ƙwayar ƙwayar cuta.Don haka, matakin magana na proteinase K gabaɗaya yayi ƙasa sosai.Nuna tsarin magana da nau'ikan da ke bayyana furotin K gabaɗaya yana buƙatar tsawon lokaci.
2. Ragowar pigments da acid nucleic
Hatsi mai girma yana gabatar da adadi mai yawa na pigment da sauran ragowar acid nucleic.Yana da wuya a cire waɗannan ƙazanta tare da tsari mai sauƙi na tsarkakewa, kuma hadaddun tsarkakewa yana ƙara yawan farashi kuma yana rage yawan dawowa.
3. Rashin zaman lafiya
Proteinase K ba shi da kwanciyar hankali, yana iya enzymolyze kanta, kuma yana da wuya a adana shi a tsaye a 37 ° C na dogon lokaci ba tare da wakili mai kariya ba.
4. Sauƙi don hazo
A lokacin da shirya daskare-bushe foda na proteinase K, domin tabbatar da cewa m abun ciki na proteinase K a cikin daskare-bushe foda ne babba, shi wajibi ne don ƙara daskare-bushe mai kariya wakili a babban taro, amma a lokacin da Matsakaicin furotin K ya kai 20mg/mL da sama, yana da sauƙi Tarin yana haifar da hazo, wanda ke kawo matsala mai yawa ga bushewar proteinase K tare da babban abun ciki mai ƙarfi.
5. Babban jari
Proteinase K yana da aikin protease mai ƙarfi kuma yana iya yin amfani da sauran ƙwayoyin cuta a cikin dakin gwaje-gwaje.Sabili da haka, proteinase K yana buƙatar wuraren samarwa na musamman, kayan aiki, da ma'aikata don bincike da haɓakawa da samarwa.
XD BIOCHEM's proteinase K maganin
XD BIOCHEM yana da balagagge furotin furci da tsarkakewa dandamali, kuma yana da arziki kwarewa a cikin magana da tsarkakewa na recombinant sunadaran da inganta samar da matakai.Ta hanyar saurin samar da ƙungiyar bincike da haɓaka, an shawo kan babban tsarin samar da furotin K.Abubuwan da ake fitarwa kowane wata na bushe-bushe foda ya fi 30 KG.Samfurin yana da ingantaccen aiki, ƙayyadaddun aikin enzyme mai girma, kuma babu sauran ragowar cytochrome da nucleic acid.Barka da zuwa tuntuɓar XD BIOCHEM Sami fakitin gwaji (E-mail:sales@xdbiochem.comTel: +86 513 81163739).
Hanyoyin fasaha na XD BIOCHEM sun haɗa da
Yin amfani da haɗin gwiwar plasmid-kwafi da yawa, an zaɓi nau'ikan maganganu masu girma tare da matakin magana na 8g/L, wanda ke shawo kan matsalar ƙarancin matakin furotin K.
Ta hanyar kafa tsarin tsarkakewa da yawa, mai masaukin cytochrome da ragowar nucleic acid na proteinase K an samu nasarar cire su a ƙasa da daidaitattun ƙimar.
Ta hanyar yin gwajin babban tsari na ƙirar kariya ta kariya, an zaɓi ma'aunin da zai iya adana proteinase K a 37°C.
Matsalolin allo suna shawo kan matsalar cewa proteinase K yana da sauƙin tarawa da hazo a babban taro, kuma yana shimfiɗa harsashin furotin K babban abun ciki mai daskarewa- bushewa.
XD BIOCHEM proteinase K samfurin
XD BIOCHEM proteinase K gwajin kwanciyar hankali: ba za a sami babban canji a cikin aiki ba bayan 80 d a cikin zafin jiki
XD BIOCHEM proteinase K gwajin kwanciyar hankali: ba za a sami babban canji a cikin aiki ba bayan 80 d a cikin zafin jiki.
Kwatanta tasirin hakar acid nucleic na XD BIOCHEM proteinase K da samfuran gasa.A cikin aiwatar da hakar acid nucleic, XD BIOCHEM da m proteinase K ana amfani da su bi da bi.Haɓakar haɓakar haɓakar XD BIOCHEM proteinase K ya fi girma kuma ƙimar Ct na ƙwayar da aka yi niyya ya ragu.
Lokacin aikawa: Dec-31-2021