FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS: 102286-67-9

Fluorescein mono-beta-D-galactopyranoside, kuma aka sani da FMG, wani fili ne mai kyalli wanda aka saba amfani dashi azaman madogara a cikin gwaje-gwajen nazarin halittu daban-daban da ƙwayoyin halitta.An samo shi daga methyl-beta-D-galactopyranoside ta hanyar haɗa shi tare da kwayoyin fluorescein.FMG ana amfani dashi sosai don nazarin ayyukan beta-galactosidase, wani enzyme wanda ke haifar da hydrolysis na lactose cikin galactose da glucose.Ta hanyar amfani da FMG a matsayin ma'auni, masu bincike za su iya sa ido kan ayyukan enzymatic na beta-galactosidase ta hanyar auna fitar da kyalli.Halin hydrolysis na FMG ta beta-galactosidase yana haifar da sakin fluorescein, yana haifar da karuwa a cikin siginar kyalli wanda za'a iya ƙididdige shi. Wannan fili kuma ana amfani dashi don bincikar ganewar carbohydrate da hulɗar.Ana iya amfani da FMG azaman binciken kwayoyin halitta don nazarin alaƙar alaƙar lectins (sunadarai waɗanda ke ɗaure musamman ga carbohydrates) zuwa carbohydrates mai ɗauke da galactose.Za'a iya gano ɗaurin hadaddun FMG-lectin da ƙididdigewa bisa ga canje-canje a cikin iskar haske. Gabaɗaya, FMG kayan aiki ne mai mahimmanci a cikin nazarin ayyukan enzyme da ƙwarewar carbohydrate, yana ba da hanya mai dacewa da mahimmanci don auna haske da kimanta waɗannan hanyoyin nazarin halittu.

oda ko Neman Quote

Cikakken Bayani

Tags samfurin

Aikace-aikace da Tasiri

Fluorescein mono-beta-D-galactopyranoside (FMG) kwayar halitta ce da aka saba amfani da ita a cikin binciken nazarin halittu a matsayin abin da za a iya ganowa da aiki na beta-galactosidase enzyme.FMG ya samo asali ne daga lactose na sukari kuma ana haɗe shi da kwayoyin fluorescein.

Babban tasirin FMG shine cewa beta-galactosidase ne ke sanya shi musamman, wani enzyme wanda ke rushe lactose zuwa galactose da glucose.Wannan enzymatic hydrolysis na FMG yana haifar da sakin fluorescein, wanda ke fitar da siginar haske mai ƙarfi.

Babban aikace-aikacen FMG shine ganowa da auna ayyukan beta-galactosidase a cikin samfura daban-daban.Ana samun wannan enzyme a cikin kwayoyin halitta da yawa, ciki har da ƙwayoyin cuta da ƙwayoyin dabbobi masu shayarwa, kuma ayyukansa na iya zama nuni ga matakai daban-daban na salon salula da hanyoyin rayuwa.

Ta amfani da FMG a matsayin maɗaukaki, ana iya auna ayyukan beta-galactosidase ta hanyar lura da hasken haske da ke fitowa ta ƴantaccen fluorescein.Ana iya yin wannan ma'auni a cikin saitin gwaji iri-iri, gami da gwaje-gwajen in vitro da nazarin hoton tantanin halitta.

Bugu da ƙari, za a iya amfani da FMG azaman kayan aiki don nazarin rarrabawa da ganowar beta-galactosidase a cikin sel.Ta hanyar amfani da fasahar microscopy mai kyalli, masu bincike za su iya hango hasken hasken da FMG ke fitarwa akan hydrolysis, ba su damar bin sararin samaniya da ayyukan beta-galactosidase.

Samfurin Samfura

Shirya samfur:

Ƙarin Bayani:

Abun ciki	Saukewa: C26H22O10
Assay	99%
Bayyanar	Farin foda
CAS No.	102286-67-9
Shiryawa	Karami da girma
Rayuwar Rayuwa	shekaru 2
Adana	Ajiye a wuri mai sanyi da bushewa
Takaddun shaida	ISO.