4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4

Enzyme substrates: 4NPM za a iya amfani da a matsayin substrate ga daban-daban enzymes, ciki har da glycosidases da related enzymes.Wadannan enzymes suna raba haɗin glycosidic tsakanin mannose da 4NPM, wanda ya haifar da sakin nitrophenyl moiety.Za'a iya auna girman hydrolysis na substrate ta hanyar kallo ta hanyar sa ido kan shayar da rukunin nitrophenyl da aka saki a wani takamaiman tsayin tsayi.Wannan yana bawa masu bincike damar tantance ayyukan enzyme da motsin motsi.

Gwaje-gwaje don metabolism na carbohydrate: Ta hanyar amfani da 4NPM a matsayin ma'auni, masu bincike zasu iya nazarin ayyukan enzymes da ke cikin ƙwayar carbohydrate, irin su alpha-mannosidases.Wadannan enzymes suna yin hydrolyze glycosidic bonds a cikin mahaɗan da ke ɗauke da mannose, kuma ana iya auna ayyukan su ta hanyar saka idanu akan sakin nitrophenyl moiety daga 4NPM.

Nazarin Glycosylation: 4NPM kuma ana iya amfani dashi a cikin gwaje-gwaje don bincika enzymes da ke cikin hanyoyin glycosylation.Glycosylation shine tsarin haɗa kwayoyin sukari zuwa sunadarai ko wasu kwayoyin halitta, kuma yawancin enzymes suna shiga cikin wannan tsari.Ta amfani da 4NPM azaman mai karɓa, masu bincike zasu iya auna canja wurin ƙwayar sukari zuwa 4NPM ta takamaiman enzymes da ke cikin halayen glycosylation.

Nunawa ga masu hana enzyme ko masu kunnawa: 4NPM za a iya amfani da su a cikin babban gwajin gwaje-gwaje don gano mahadi waɗanda ke hana ko kunna takamaiman enzymes.Ta hanyar yin la'akari da tasirin mahadi na gwaji akan hydrolysis ko gyare-gyare na 4NPM ta hanyar enzymes da aka yi niyya, masu bincike zasu iya gano yiwuwar magungunan warkewa ko binciken sinadarai masu amfani don nazarin aikin enzyme.