2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

Enzyme Substrate: ONPG yawanci ana amfani dashi azaman madaidaicin beta-galactosidase, wani enzyme wanda ke sarrafa ONPG don samar da fili mai launin rawaya (o-nitrophenol) wanda za'a iya gano shi cikin sauƙi ta hanyar hoto.Ana iya amfani da wannan amsawar enzymatic don auna ayyukan beta-galactosidase, yin ONPG kayan aiki mai mahimmanci a cikin karatun enzymology.

Ƙididdigar ilmin halitta ta kwayoyin halitta: ONPG ana amfani da shi azaman maƙasudi a cikin gwaje-gwajen nazarin halittu daban-daban, musamman a cikin binciken kwayoyin halitta na beta-galactosidase.A cikin waɗannan gwaje-gwajen, ana amfani da madaidaicin tushen ONPG don auna ayyukan jigilar mai ba da rahoto, wanda yawanci ana tsara shi ta wani jerin abubuwan sha'awa na masu talla.Ayyukan beta-galactosidase, wanda canjin launi ya nuna akan ONPG hydrolysis, yana ba da bayanai masu mahimmanci game da ayyukan mai talla.

Binciken maganganun Gene: ONPG kuma ana amfani dashi a cikin nazarin maganganun kwayoyin halitta.Ta hanyar haɗa jerin masu tallata sha'awa zuwa jigon beta-galactosidase, masu bincike za su iya auna ayyukan beta-galactosidase ta amfani da ONPG a matsayin ƙasa.Matsayin aikin beta-galactosidase yana nuna ƙarfi da aiki na mai gabatarwa, yana ba da damar kimanta matakan maganganun kwayoyin halitta.

Aikace-aikacen bincike: Ana iya amfani da ONPG a aikace-aikacen bincike, musamman a cikin gano ƙwayoyin cuta.Kwayoyin cuta daban-daban, irin su Escherichia coli da wasu nau'in Shigella da Salmonella, suna samar da beta-galactosidase wanda zai iya raba ONPG.Wannan halayen hydrolysis yana haifar da canjin launi mai gani, wanda za'a iya amfani dashi don sanin kasancewar waɗannan kwayoyin cutar a cikin samfurori na asibiti.