Tris (hydroxymethyl) nitromethane CAS: 126-11-4

Tasiri:

Ƙarfin Buffering: Tris yana aiki azaman ingantacciyar wakili na buffer saboda ikonsa na karɓa ko ba da gudummawar protons, yana kiyaye tsayayyen kewayon pH a cikin mafita.Ana amfani da shi azaman babban sashi a cikin tsarin buffer don daidaita pH na samfuran halitta da halayen halitta.

Aikace-aikace:

Halittar Halitta: Ana amfani da Tris a matsayin wakili mai ɓoyewa a cikin dabaru daban-daban na ilimin halitta, gami da DNA da keɓewar RNA, PCR, gel electrophoresis, da tsarkakewar furotin.Yana taimakawa wajen kiyaye yanayin pH mai tsayi, yana ba da damar yanayi mafi kyau don halayen enzymatic da hulɗar kwayoyin halitta.

Al'adun Salula: Ana amfani da Tris sau da yawa a cikin kafofin watsa labarai na al'adun sel don kiyaye pH akai-akai da ma'aunin osmotic, yana tallafawa haɓakar ƙwayoyin lafiya da yuwuwa.

Chemistry na Protein: Ana amfani da Tris a cikin gwaje-gwajen sunadarai na sunadaran, irin su furotin solubilization, ƙididdigar kwanciyar hankali na furotin, da kuma nazarin ɗaurin furotin.Yana taimakawa wajen kiyaye kewayon pH da ake so, yana tabbatar da nadawa da aiki mai kyau.

Enzymology: Ana amfani da Tris a cikin gwaje-gwajen enzymatic daban-daban don inganta yanayin pH da ake buƙata don ayyukan enzymatic.Yana tabbatar da abin dogara da sakamakon da za a iya sakewa, yana ba da damar ma'auni daidai na kinetics na enzyme da nazarin hanawa.

Gwaje-gwajen Kwayoyin Halitta: Ana amfani da Tris a matsayin wani sashi a yawancin gwaje-gwajen sinadarai saboda abubuwan da ke tattare da shi.Yana kula da pH akai-akai a lokacin launi, spectrophotometric, da enzymatic assays, haɓaka daidaito da amincin sakamakon.