TRIS-Acetate CAS: 6850-28-8 Farashin Mai samarwa

Tris-acetate (TRIS-Acetate) wani abu ne da aka saba amfani dashi a cikin gwaje-gwajen halittu da na halitta.Ya ƙunshi haɗin tris (hydroxymethyl) aminomethane (Tris) da acetic acid, wanda ke aiki a matsayin mai sarrafa pH da stabilizer.Matsakaicin pH na TRIS-Acetate buffer yawanci jeri daga 7.4 zuwa 8.4.
Babban tasirin TRIS-Acetate shine don kiyaye tsayayyen pH, wanda ke da mahimmanci ga yawancin halayen halittu da kwayoyin halitta.Yana aiki azaman mai ɗaukar hoto ta hanyar rage duk wani muhimmin canje-canje a cikin pH wanda zai iya faruwa saboda ƙarin acid ko tushe yayin hanyoyin gwaji.
TRIS-Acetate yana samun aikace-aikace daban-daban a cikin ilmin kwayoyin halitta, ilmin halitta, da ilimin halittu:
DNA da RNA Electrophoresis: TRIS-Acetate ana yawan amfani dashi azaman buffer mai gudana a cikin agarose da polyacrylamide gel electrophoresis.Yana ba da ingantaccen yanayin pH yayin rabuwar DNA da gutsuttsuran RNA dangane da girman su.
Protein Analysis: Ana amfani da buffers TRIS-Acetate don furotin electrophoresis, kamar SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis).Yana tabbatar da kwanciyar hankali na furotin da rabuwa yayin aiwatarwa.
Halayen Enzyme: Ana amfani da buffers na TRIS-Acetate akai-akai a cikin gwaje-gwajen enzyme da karatu.Yana ba da mafi kyawun kewayon pH don halayen enzymatic daban-daban kuma yana taimakawa kula da ayyukan enzyme.
Al'adun Kwayoyin Halitta da Nama: Ana amfani da buffers TRIS-Acetate a cikin kafofin watsa labaru na al'ada don kula da pH mai dacewa don ci gaban kwayar halitta da yaduwa.Yana taimakawa wajen kula da yanayin ilimin halittar jiki da ake buƙata don haɓakar tantanin halitta.