PIPES CAS: 5625-37-6 Farashin Mai ƙira

PIPES (piperazine-1,4-bisethanesulfonic acid) wani fili ne na buffering na zwitterionic da farko da aka yi amfani da shi a cikin binciken nazarin halittu da kwayoyin halitta.Yana da fasali da aikace-aikace masu mahimmanci da yawa, gami da:

Wakilin buffering pH: PIPES ingantaccen buffer ne wanda ke taimakawa kiyaye tsayayyen kewayon pH a cikin gwaje-gwajen halittu daban-daban.Ana yawan amfani dashi a cikin kafofin watsa labarai na al'adun sel, ƙididdigar enzyme, da aikace-aikacen ilimin halitta.

Babban ƙarfin buffering: PIPES yana da kyakkyawan ƙarfin buffer a cikin kewayon pH na 6.1 zuwa 7.5, yana sa ya dace don kiyaye yanayin pH mai tsayi a cikin kewayon tsarin ilimin halitta.

Ƙananan hulɗa tare da kwayoyin halitta: PIPES an san shi don ƙananan tsangwama tare da tsarin sinadarai da ƙananan ɗaure ga sunadarai da enzymes, yana mai da shi manufa don kiyaye mutunci da ayyukan biomolecules.

Ya dace da kididdigar dogaro da zafin jiki: PIPES na iya riƙe kaddarorin sa na buffer akan kewayon zafin jiki mai faɗi, gami da yanayin yanayin jiki da haɓaka.Wannan ya sa ya dace da gwaje-gwajen da ke buƙatar kwanciyar hankali da daidaito a yanayin yanayin zafi daban-daban.

Aikace-aikacen Electrophoresis: Ana amfani da PIPES a matsayin mai ɗaukar hoto a cikin fasahar gel electrophoresis, irin su RNA ko DNA agarose gel electrophoresis, saboda ƙarancin ƙarancin UV da kaddarorin gudanarwa.

Ƙirƙirar ƙwayoyi: Hakanan ana amfani da PIPES a cikin ƙirar magunguna azaman wakili mai ɓoyewa, yana ba da kwanciyar hankali da kiyaye mafi kyawun pH don tasirin magani.