piperazine-1,4-bis (2-ethanesulfonic acid) gishiri disodium CAS: 76836-02-7

Tasiri:

Abubuwan Buffering: Ana iya amfani da PIPES don kiyaye matakan pH akai-akai a cikin kewayon takamaiman, kamar yadda yake da tasiri a buffering a cikin kewayon pH na jiki na 6.1-7.5.Wannan yana sa ya zama mai amfani a cikin gwaje-gwajen halittu daban-daban inda sarrafa pH ke da mahimmanci.

Kwanciyar hankali: PIPES yana da ƙarfi akan yanayin zafi da yawa, yana sa ya dace da amfani a gwaje-gwajen da aka yi a ƙarƙashin yanayi daban-daban.

Aikace-aikace:

Al'adar salula: Ana iya amfani da PIPES azaman ma'auni a cikin dabarun al'adun tantanin halitta, kamar kiyaye pH na kafofin watsa labarai ko buffers da ake amfani da su don haɓakar tantanin halitta da kiyayewa.

Protein da nazarin enzyme: Ana amfani da PIPES da yawa a cikin furotin da nazarin enzyme don kula da pH mai tsayi a lokacin halayen daban-daban, musamman ma wadanda suka shafi enzymes masu mahimmanci ko sunadarai waɗanda canje-canjen pH zasu iya shafa.

Electrophoresis: Ana iya amfani da PIPES azaman buffer a cikin aikace-aikacen electrophoresis gel, yana taimakawa wajen kula da yanayin pH mafi kyau don DNA ko rabuwar furotin.

Dabarun ilimin halitta: Ana iya amfani da PIPES azaman maƙasudi a cikin dabaru daban-daban na ilimin halitta, gami da hakar DNA/RNA, PCR, da jerin DNA, tabbatar da ingantattun sakamako ta hanyar kiyaye yanayin pH.