Phenylgalactoside CAS: 2818-58-8

Tasiri akan ayyukan enzyme: Phenylgalactoside yawanci ana amfani dashi don auna aikin β-galactosidase enzyme.Lokacin da phenylgalactoside ya zama hydrolyzed ta β-galactosidase, yana sakin p-nitrophenol.Ana iya auna tarin p-nitrophenol da ƙima, yana ba da haske game da ayyukan β-galactosidase.Ana amfani da wannan tasirin a cikin aikace-aikace kamar ƙididdigar enzyme da tsarin nunawa.

Binciken maganganun Halittu: Ana amfani da Phenylgalactoside akai-akai azaman maƙasudi a cikin gwaje-gwajen ilimin halitta don nazarin maganganun kwayoyin halitta.Halin lacZ, wanda ke ɓoye β-galactosidase, yawanci ana haɗa shi tare da jerin ka'idoji na sauran kwayoyin halitta masu sha'awa.Maganar kwayar halittar lacZ da hydrolysis na phenylgalactoside ta β-galactosidase na iya nuna alamar magana da matakin jigon da aka yi nazari.

Tsarin nunawa: Phenylgalactoside muhimmin sashi ne na tsarin nunawa wanda ke amfani da ayyukan β-galactosidase.Misali daya da aka fi sani da shi shine hanyar tantance shudi-fari, wacce ake amfani da ita don gano recombinant ko canza sel a gwaje-gwajen ilmin kwayoyin halitta.Mallaka waɗanda suka sami nasarar ɗaukar DNA ɗin sake haɗawa ko kuma sake haɗuwa da ƙwayoyin halitta za su bayyana β-galactosidase, wanda ke haifar da hydrolysis na phenylgalactoside da samuwar launin shuɗi.

Tsabtace sunadaran: A wasu lokuta, ana iya amfani da phenylgalactoside azaman ligand don chromatography na alaƙa don tsarkake sunadaran da ke ɗaure musamman ko suna kunna ta β-galactosidase.Sunan furotin na sha'awa na iya samun alamar alaƙa ko alamar haɗin kai mai ƙunshe da yanki mai ɗaure β-galactosidase.Ta hanyar wuce cakuda furotin ta hanyar ginshiƙi tare da phenylgalactoside mara motsi, ana iya zaɓar furotin da ake so kuma daga baya ya ƙare.