Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactpyranoside CAS:24404-53-3

Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ana amfani dashi a matsayin ma'auni don enzyme β-galactosidase.Lokacin da β-galactosidase ya yi aiki a kai, mahallin yana yin hydrolyzed, yana haifar da samar da p-nitrophenol ko o-nitrophenol, wanda ke da launin rawaya wanda za'a iya ganowa cikin sauƙi.Wannan yana sa ya zama mai amfani azaman maƙasudin ƙididdiga waɗanda ke auna ayyukan β-galactosidase.

Ɗaya daga cikin aikace-aikacen gama gari na Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside yana cikin ƙididdigar kwayoyin halitta.A cikin waɗannan gwaje-gwajen, an haɗa nau'in jinsin sha'awa zuwa jinsin mai ba da rahoto, kamar kwayar beta-galactosidase.Sa'an nan kuma ana kula da aikin jigilar mai ba da rahoto ta hanyar auna aikin β-galactosidase ta amfani da Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside a matsayin substrate.Wannan yana ba masu bincike damar yin nazarin maganganun kwayoyin halitta da ka'idoji.

Hakanan za'a iya amfani da Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside don tantance masu hanawa ko masu kunnawa na β-galactosidase.Ta hanyar gwada mahadi daban-daban ko masu neman magani, masu bincike zasu iya gano ƙwayoyin da ke daidaita ayyukan wannan enzyme, waɗanda zasu iya samun aikace-aikacen warkewa a wasu cututtuka ko yanayi.

Bugu da ƙari, ana iya amfani da wannan fili a cikin tsarkakewar furotin.β-galactosidase galibi ana haɗa su da sunadaran sha'awa don sauƙaƙe gano su da tsarkakewa.Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside za a iya amfani dashi a matsayin substrate don nunawa da kuma tsarkake waɗannan sunadaran haɗin gwiwa ta hanyar gano ayyukan β-galactosidase.