IPTG CAS: 367-93-1 Farashin Mai ƙira
Isopropyl β-D-1-thiogalactopyranoside (IPTG) wani analog na roba ne na lactose wanda aka saba amfani dashi a cikin binciken nazarin halittun kwayoyin halitta da aikace-aikacen fasahar halittu.Ana amfani da IPTG da farko don haifar da bayyanar da kwayoyin halitta a cikin tsarin kwayoyin cuta, inda yake aiki a matsayin abin jawo kwayoyin halitta don fara rubutun kwayoyin halitta.
Lokacin da aka ƙara zuwa matsakaicin girma, IPTG ana ɗauka ta hanyar ƙwayoyin cuta kuma yana iya ɗaure da furotin na lac repressor, yana hana shi toshe ayyukan lac operon.Lac operon wani gungu ne na kwayoyin halitta da ke da hannu a cikin metabolism na lactose, kuma lokacin da aka cire furotin mai hanawa, ana bayyana kwayoyin.
Ana amfani da IPTG sau da yawa tare tare da mai tallata mutant lacUV5, wanda shine nau'in mai tallata lac.Ta hanyar haɗa shigar da IPTG tare da wannan mai tallata mutant, masu bincike za su iya cimma manyan matakan bayyana kwayoyin halitta.Wannan yana ba da damar samar da adadi mai yawa na furotin don tsarkakewa ko wasu aikace-aikace na ƙasa.
Bugu da ƙari ga maganganun kwayoyin halitta, ana kuma amfani da IPTG akai-akai a cikin gwaje-gwajen gwajin shuɗi/ fari.A cikin wannan fasaha, ƙwayar lacZ yawanci tana haɗawa da jinsin sha'awa, kuma ƙwayoyin cuta waɗanda suka sami nasarar bayyana wannan haɗin gwiwar za su samar da enzyme β-galactosidase mai aiki.Lokacin da aka ƙara IPTG tare da chromogenic substrate kamar X-gal, ƙwayoyin cuta waɗanda ke bayyana jigon fusion sun zama shuɗi saboda ayyukan β-galactosidase.Wannan yana ba da damar ganowa da zaɓin nau'ikan nau'ikan sake haɗawa waɗanda suka sami nasarar haɗa kwayar halittar sha'awa.
Ƙaddamar da maganganun kwayoyin halitta: IPTG ana yawan amfani dashi don haifar da bayyanar da kwayoyin halitta a cikin tsarin kwayoyin cuta.Yana kwaikwayon lactose inducer na halitta kuma yana ɗaure ga furotin na lac repressor, yana hana shi toshe lac operon.Wannan yana ba da damar rubutawa da bayyana abubuwan da ake so.
Maganar furotin da tsarkakewa: Ana amfani da shigar da IPTG sau da yawa don samar da adadi mai yawa na sunadaran sake haɗewa don dalilai daban-daban, kamar nazarin sinadarai, samarwa na warkewa, ko nazarin tsari.Ta hanyar amfani da ma'anar maganganun da suka dace da shigar da IPTG, masu bincike za su iya cimma babban matakan samar da furotin da aka yi niyya a cikin rundunonin ƙwayoyin cuta.
Blue/fari nunawa: Ana amfani da IPTG akai-akai a hade tare da kwayoyin lacZ da chromogenic substrate, irin su X-gal, don gwaje-gwajen gwajin shuɗi / fari.Halin lacZ yawanci yana hade da jinsin sha'awa, kuma kwayoyin cutar da suka sami nasarar bayyana wannan kwayar halittar za su samar da enzyme β-galactosidase mai aiki.Lokacin da aka ƙara IPTG da chromogenic substrate, nau'ikan sake haɗawa da ke bayyana jigon ƙullun ya zama shuɗi, yana ba da damar ganowa da zaɓi cikin sauƙi.
Nazarin ka'idojin kwayoyin halitta: Ana amfani da shigar da IPTG a cikin bincike don nazarin ka'idojin kwayoyin halitta da operons, musamman lac operon.Ta hanyar sarrafa adadin IPTG da sa ido kan maganganun lac operon, masu bincike za su iya bincika hanyoyin sarrafa kwayoyin halitta da kuma rawar abubuwa daban-daban ko maye gurbi.
Tsarin maganganu na Gene: IPTG wani muhimmin abu ne a cikin tsarin maganganun kwayoyin halitta da yawa, kamar tsarin tushen T7.A cikin waɗannan tsarin, ana amfani da mai haɓaka lac sau da yawa don fitar da furcin T7 RNA polymerase, wanda, bi da bi, yana rubuta kwayoyin da aka yi niyya a ƙarƙashin kulawar jerin masu tallata T7.Ana amfani da IPTG don haifar da bayyanar T7 RNA polymerase, wanda ke haifar da kunna maƙasudin maganganu.
| Abun ciki | Saukewa: C9H18O5S |
| Assay | 99% |
| Bayyanar | Farin foda |
| CAS No. | 367-93-1 |
| Shiryawa | Karami da girma |
| Rayuwar Rayuwa | shekaru 2 |
| Adana | Ajiye a wuri mai sanyi da bushewa |
| Takaddun shaida | ISO. |
