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HEPES CAS: 7365-45-9 Farashin Mai samarwa

HEPES (4- (2-Hydroxyethyl) piperazine-1-ethanesulfonic acid) wani buffer ne na halitta wanda aka saba amfani dashi a cikin binciken nazarin halittu da sinadarai.An san shi don ikonsa don kula da pH mai tsayi a cikin mafita mai ruwa, yana sa ya dace da aikace-aikace daban-daban da suka shafi tsarin salula da enzymatic.HEPES wani fili ne na zwitterionic, ma'ana yana da ƙididdiga masu kyau da mara kyau akan ƙungiyoyi masu aiki daban-daban, wanda ya ba shi damar yin tsayayya da canje-canje a cikin pH wanda ya haifar da ƙari na acid ko tushe.Ana amfani dashi akai-akai a cikin al'adun tantanin halitta, ƙididdigar enzymes, nazarin furotin, gwaje-gwajen electrophoresis, da ƙirar magunguna.


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Aikace-aikace da Tasiri

Buffering pH: HEPES ana amfani dashi ko'ina azaman wakili na buffer don kiyaye tsayayyen pH a cikin kafofin watsa labarai na al'adar tantanin halitta da ƙididdigar nazarin halittu.Yana aiki yadda ya kamata a cikin kewayon pH na physiological na 6.8 zuwa 8.2, yana sa ya dace da kiyaye mafi kyawun yanayi don hanyoyin nazarin halittu.

Al'adar salula: Ana ƙara HEPES zuwa kafofin watsa labarai na al'ada don daidaita matakin pH, samar da yanayi mai dacewa don haɓakar tantanin halitta da kiyaye iyawar tantanin halitta.Yana taimakawa hana haɓakar pH wanda zai iya haifar da mummunan tasiri akan halayen sel da sakamakon gwaji.

Ƙididdigar Enzyme: Ana amfani da HEPES sau da yawa a matsayin mai ɓoyewa a cikin halayen enzymatic saboda ikonsa na kula da takamaiman pH.Yana taimakawa wajen kiyaye mafi kyawun aikin enzymatic da kwanciyar hankali, don haka haɓaka daidaito da amincin ƙididdigar enzymes.

Nazarin furotin: Ana amfani da HEPES a cikin gwaje-gwaje masu alaƙa da furotin daban-daban, gami da tsarkakewar furotin, crystallization na furotin, da nazarin tsarin furotin.Yana taimakawa wajen daidaita pH da kula da daidaitaccen nadawa da kwanciyar hankali yayin waɗannan gwaje-gwajen.

Electrophoresis: HEPES yana samun aikace-aikace a cikin fasahar gel electrophoresis, kamar SDS-PAGE da agarose gel electrophoresis.Ana amfani da shi azaman wakili na buffer a cikin buffer mai gudana, yana samar da ingantaccen yanayin pH don rabuwa da nazarin kwayoyin halitta.

Samfuran Magunguna: Ana amfani da HEPES a cikin ƙirƙira samfuran magunguna da fasahar kere-kere.Yana taimakawa wajen kiyaye kwanciyar hankali da amincin kayan aiki masu aiki, yana tabbatar da ingancin su da rayuwar shiryayye.

Shirya samfur:

6892-68-8-3

Ƙarin Bayani:

Abun ciki Saukewa: C8H18N2O4S
Assay 99%
Bayyanar Farin foda
CAS No. 7365-45-9
Shiryawa Karami da girma
Rayuwar Rayuwa shekaru 2
Adana Ajiye a wuri mai sanyi da bushewa
Takaddun shaida ISO.

 


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