5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) wani abu ne na chromogenic da aka saba amfani dashi don gano ayyukan beta-glucuronidase (GUS).GUS wani enzyme ne da ake samu a cikin halittu daban-daban, gami da kwayoyin cuta, tsirrai, da dabbobi masu shayarwa.Ana amfani da X-Gluc akai-akai a cikin ƙididdigar masu ba da rahoto na GUS da binciken ilimin halitta.

Babban aikace-aikacen X-Gluc yana cikin dabarun ɓacin rai na histochemical, inda zai iya hango yanayin magana da ayyukan GUS enzyme.Wannan sinadari mai iya jujjuyawar tantanin halitta kuma ya zama ruwa ta hanyar GUS, wanda ke haifar da samuwar hazo mai shuɗi ko samfur mara narkewa.Wannan launin shudi yana ba masu bincike damar ganowa da gano ayyukan GUS a cikin sel, kyallen takarda, da dukkan kwayoyin halitta.

Hakanan ana iya amfani da X-Gluc a cikin ƙididdiga masu ƙima don auna ayyukan enzyme na GUS.Ƙarfin launin shuɗi ko adadin samfurin da aka kafa za a iya danganta shi da matakin GUS ko aikin enzymatic.

Bugu da ƙari, an yi amfani da X-Gluc a cikin binciken kwayoyin halitta don nazarin maganganun kwayoyin halitta, ayyukan masu tallatawa, da kuma canza shuka.Hakanan an yi amfani dashi a cikin tsarin ƙwayoyin cuta don cloning da gano sunadaran haɗin GUS.