3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE CAS:3150-25-2

Gano ayyukan beta-galactosidase: Ana amfani da ONPG sau da yawa don tantance kasancewar da ayyukan beta-galactosidase a cikin samfuran halitta daban-daban, kamar al'adun ƙwayoyin cuta ko ƙwayoyin sel.Samar da o-nitrophenol, wanda ke da launin rawaya, ana iya auna shi cikin sauƙi ta amfani da spectrophotometer.

Nazarin magana akan Gene: ONPG ana amfani da shi sosai a cikin binciken nazarin halittu don nazarin maganganun kwayoyin halitta.Ta hanyar haɗa mai tallan kwayar halitta mai ban sha'awa tare da kwayar halittar beta-galactosidase, masu bincike za su iya auna ayyukan wannan mai talla ta ƙara ONPG da ƙididdige sakamakon samar da o-nitrophenol.Wannan hanya, wanda aka sani da beta-galactosidase reporter assay, yana ba da bayani game da aikin rubutun kwayoyin halitta.

Ganewar ƙwayoyin cuta: Wasu ƙwayoyin cuta suna samar da beta-galactosidase, yayin da wasu ba sa.Ana iya amfani da ONPG a haɗe tare da wasu gwaje-gwajen sinadarai don gano nau'in ƙwayoyin cuta dangane da ikonsu na yin ruwa na ONPG.Ana yawan amfani da wannan hanyar a cikin bincike na asibiti da dakunan gwaje-gwajen microbiology.

Nunawa don masu hana enzyme ko masu kunnawa: ONPG za a iya amfani da su don nunawa ga mahaɗan da ke daidaita ayyukan beta-galactosidase.Ta hanyar auna aikin enzyme a gaban mahaɗan daban-daban, masu bincike za su iya gano masu hana masu hanawa ko masu kunnawa waɗanda za a iya ƙarin bincike don yiwuwar maganin su.